[Histonet] Re: Inking esophagus biopsies

[Langley, Kristi L.]

Do people still use hematoxilyn? I went from one lab using safranin to now using hematox....I can not get them to switch either...in my opinion safranin works so much better, but they are not open to any changes of any kind......:( and if I offer any I am even told I am questioning authority..Help me find documentation to prove my case

Kristi Langley HT(ASCP)cm

St. Luke's Medical Center

2720 Stone Park Blvd.

Sioux City, Ia 51104

712-279-3164 ext 6

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Bob Richmond [rsrichmond <@t> gmail.com]
Sent: Wednesday, December 12, 2012 12:17 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Inking esophagus biopsies

Sheila Adey (somewhere in Canada) asks: Can anyone reccommend a good
way to ink esophageal biopsies without using mercurochrome?

Out of many pathology services I've worked on, I've only seen one that
marked GI biopsies when grossing. They used safranin O (the routine
Gram stain counterstain, from the microbiology lab) and it worked
quite well.

I would not use eosin because it makes fluorescence microscopy
impossible. You can't get mercurochrome any more (in the US anyway),
and you don't want the mercury in your processor anyway.

Bob Richmond
Samurai Pathologist
Maryville TN

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