[Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDEDBLOCKS

[Lee & Peggy Wenk]

Can you surface decal?

After facing the block on the microtome, getting a full face, pour some 
decalcification fluid in the lid of a coplin jar. Place the block faced side 
down in the decal solution, for about 30 minutes.

Rinse the acid off the block with some cool water (don't want acid dripping 
on the microtome blade holder and blade). Line the block up exactly on the 
microtome to the knife. The first 2-4 sections will be decalcified enough 
for you to cut. (That's why the block has to be lined up exactly - can't 
waste rough trimming off the 10-20 um of decalcified tissue.)

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Health System
Royal Oak, MI 48073

The opinions expressed are mine, and do not reflect on my place of 
employment.

-----Original Message----- 
From: Fiona J Morrow
Sent: Thursday, February 28, 2013 8:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN 
EMBEDDEDBLOCKS

Hi

Has anyone ever tried to reprocess bone tissue that has been under
decalcified and processed through to wax?

Thanks

Fi M


Fiona Morrow
Dept. of Infection and Immunity
KFloor, Room K118
Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX

0114 271 2102
F.morrow <@t> sheffield.ac.uk

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet