[Histonet] pepsin pre-digestion before immuno staining

[Amy Porter]

Disregard my previous email that was for Trypsin.....duh, sorry about that!!
Pepsin we use at a 0.04% in 0.2N HCl for 20 minutes at 37C

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils,
Paul
Sent: Thursday, June 27, 2013 2:59 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] pepsin pre-digestion before immuno staining

We use trypsin and protease fairly often in our lab in conjunction with
immuno staining, but we have not used pepsin.  An investigator wants us to
use pepsin, and we do have some, but would appreciate any recommendations
regarding its use - concentration, time, temperature.
Thanks

 

Paul M.

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