[Histonet] pepsin pre-digestion before immuno staining

Amy Porter portera <@t> msu.edu
Thu Jun 27 14:32:17 CDT 2013

0.1% Trypsin + 0.1% Calcium Chloride in PBS for 10-20 minutes at 37C

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils,
Sent: Thursday, June 27, 2013 2:59 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] pepsin pre-digestion before immuno staining

We use trypsin and protease fairly often in our lab in conjunction with
immuno staining, but we have not used pepsin.  An investigator wants us to
use pepsin, and we do have some, but would appreciate any recommendations
regarding its use - concentration, time, temperature.


Paul M.

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