[Histonet] Mixing Paraffin Brands

[DiCarlo, Margaret]

I use Tissue Prep 2 for human bones and have previously used it for mice bone joints.  It works very well for both.

Peggy DiCarlo


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Louro, Pedro
Sent: Wednesday, June 19, 2013 14:33
To: Rene J Buesa; Lucie Guernsey; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Mixing Paraffin Brands

Just out of curiosity, what paraffin would people out there recommend using for animal bone joints and turbinates?
We are currently using parapalst plus and was thinking of changing to a "harder paraffin"

Any thoughts???

Pedro L.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, June 19, 2013 2:24 PM
To: Lucie Guernsey; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Mixing Paraffin Brands

Each paraffin has some additives to improve either its penetration rate or density to section.
Mixing different melting points (MP) paraffin will result in another paraffin with an intermediate MP and the sectioning will be different.
Preparing the block with the mixture will probably cause so troubles while sectioning.
Why don't you just use them separate? There is no good reason to mix them and the two paraffins you mention are of good quality.
René J.

From: Lucie Guernsey <lguernsey <@t> ucsd.edu>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Wednesday, June 19, 2013 2:01 PM
Subject: [Histonet] Mixing Paraffin Brands


Hi,

Does anyone know if there's a reason why one shouldn't mix different brands
of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56
C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point
52 C). Will the different melting points be a problem?

If we were to use the McCormick paraffin, the only place it may mix with
the Fisherbrand paraffin is in the blocks themselves (as we refill the
embedder). But I don't want to compromise the quality of our blocks just to
not waste the free paraffin.

Or, another option could be that we use the McCormick in the processor and
the Fisherbrand in the embedder. Could that cause issues in the blocks as
the tissue would be infiltrated with one brand and embedded in another?

Maybe I'm over-thinking this......

Many thanks!
Lucie

Lucie Guernsey
UCSD
Dept. of Pathology
lguernsey <@t> ucsd.edu
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