[Histonet] Sakura Tissue-Tek Prisma
Casey Arnold
carnold <@t> ourlab.net
Thu Jun 13 08:15:23 CDT 2013
We have recently started using a Sakura Tissue-tek Prisma that we inherited
from our sister lab. While running some validation testing I have noticed
that the staining is very inconsistent. The tissue will stain from light to
dark within the same rack and sometimes within the same cut (slide). I have
reset the mix, speed, etc, to factory settings. I have tried increasing my
hematoxylin, and bluing times. I have changed water filters, decreased
water rinse times, tried different types of tissue, all to no avail. Has
anyone had this problem or have any suggestions on how to correct it? I
have tried everything the Sakura tech line has recommended and am still at
a loss.
--
NOTICE: This electronic mail message and any files transmitted with it are
intended exclusively for the individual or entity to which it is addressed.
The message, together with any attachment, may contain confidential and/or
privileged information. Any unauthorized review, use, printing, saving,
copying, disclosure or distribution is strictly prohibited. If you have
received this message in error, please immediately advise the sender by
reply email and delete all copies.
Thank you
*Casey Arnold
*
*Histology Supervisor**
OURLabs / OPKO Diagnostics, LLC*
1450 Elm Hill Pike
Nashville, TN 37210
Direct: 615-345-4582
Office: 615-874-0410
Fax: 615-345-4595
More information about the Histonet
mailing list