[Histonet] Paraffin processing native sheep ACL

[Elizabeth Ronan]

Hello,

I need to paraffin process native sheep anterior cruciate ligament (ACL) 
that has been fixed in 10% neutral buffered formalin for 7 days. I was 
wondering if anyone with more expertise on this subject could guide me 
with the best lengths of times in the alcohols, xylenes, and paraffin to 
fix ACL. I tried a 12 hour program but the sections crumbled in the 
middle and it appeared that the paraffin had not fully perfused the 
ligament.

I have access to the following program, and can alter the lengths of the 
steps for as long as desired:

Program:
70%
80%
95%
95%
100%
100%
Xylene
Xylene
Paraffin
Paraffin
Paraffin

Any advice is much appreciated.
Thanks for your time,
Liz