[Histonet] RE: Tissue Falling off

[Smith, Allen]

Use silane-treated slides: buy them of make your own with "Vectabond" or something similar.  I have only once in 6 years lost a section off a silane-treated slide.
- Allen A. Smith, Ph.D.
  Barry University School of Podiatric Medicine
  Miami Shores, Florida

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF
Sent: Thursday, June 06, 2013 10:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tissue Falling off

Good Morning,
I have a question about tissue falling off in the higher ph decloaking.     I just tried using 10%NBF for 20 minutes after deparaffinizing and before decloaking.  I still had the tissue fall off.  It is well fixed breast tissue.  Could I use albumin on the slide or will that interfere with the staining?    Any suggestions welcomed or if you have another way of doing the formalin trick.    I use a pressure cooker and it gets up to 126.5c for 5 minutes and then goes down to 89.5c for ten seconds.

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford <@t> dignityhealth.org
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