[Histonet] Tissue Falling off

[Rene J Buesa]

And why would you use 10% NBF after deperaffinizing and before decloaking?
Use (+) charged slides
René J.

From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> DignityHealth.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Thursday, June 6, 2013 10:12 AM
Subject: [Histonet] Tissue Falling off


Good Morning,
I have a question about tissue falling off in the higher ph decloaking.    I just tried using 10%NBF for 20 minutes after deparaffinizing and before decloaking.  I still had the tissue fall off.  It is well fixed breast tissue.  Could I use albumin on the slide or will that interfere with the staining?    Any suggestions welcomed or if you have another way of doing the formalin trick.    I use a pressure cooker and it gets up to 126.5c for 5 minutes and then goes down to 89.5c for ten seconds.

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford <@t> dignityhealth.org
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