[Histonet] Histochoice MB vs other fixatives
yyung <@t> scripps.edu
Mon Jul 29 18:00:27 CDT 2013
Does anyone have experience using Histochoice for IHC, in particular for mouse brain tissue? Cryoprotected vs paraffinized material? I am currently looking at both structural proteins (e.g., tubulin) as well as transcription factors (e.g., b-catenin and Pax6). I normally use 4% PFA (cryoprotected, frozen, OCT embedded) FAA (formalin-ethanol-acetic acid) (paraffin embedded). These two fixes work decently, but now I'm starting to do IHC for G protein-coupled receptors and some have suggested using PLP as an alternative.
There was a small discussion in 2009 on this list; hopefully more people have experience with it now.
Yun C. Yung, Ph.D.
Chun Lab, Dorris Neuroscience Center
The Scripps Research Institute
10550 N. Torrey Pines Rd, DNC-118
La Jolla, CA 92037
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