[Histonet] Embedding Contamination

Jay Lundgren jaylundgren <@t> gmail.com
Wed Jul 24 17:44:50 CDT 2013

     Everyone jumps on the embedder in these situations, but the
contamination might be coming from the gross board or the  tissue processor
as well.  So be sure to train the appropriate docs/PA's/histotechs how to
avoid cross contamination when grossing/processing as well.  And stop
jumping on the embedder, 'cause the dirt from your shoes might be what's
causing the contamination in the first place.


                Jay A. Lundgren, M.S., HTL (ASCP)

On Wed, Jul 24, 2013 at 11:54 AM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> This how I always handled this issue:
> 1- document the contamination in your QC file trying to identify the
> tissue source;
> 2- train the embedding histotechs in the proper way of cleaning the
> embedding instruments and the forceps wells and document it also in the
> corresponding QC file;
> 3- save the slide marking the contaminating tissue fragment;
> 4- remove the contaminant and recut the block. Do not melt the block down,
> just remove the contaminant with a needle.
> 5- the slide to be filed with the case is the recut (step 4).
> Probably other people handle this issue differently, but that is how I
> handled it
> René J.
> From: Roger Heyna <rheyna <@t> lumc.edu>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Wednesday, July 24, 2013 11:05 AM
> Subject: [Histonet] Embedding Contamination
> Hi Histonetters:
> When a pathologist identifies extraneous tissue on a slide, and histology
> determines that the tissue contamination originates from embedding, what is
> your procedure for correcting the embedding? Do you remove the extraneous
> (or contamination) tissue from the block, recut the slide, and discard the
> original contaminated slide? Do you leave the extraneous tissue in the
> block and make a note in the report and/or on the slide? Do you keep track
> of these in a log somewhere?
> Thanks in advance for your help.
> Roger
> Maywood, IL
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