[Histonet] Embedding Contamination

Rene J Buesa rjbuesa <@t> yahoo.com
Wed Jul 24 11:54:53 CDT 2013

This how I always handled this issue:
1- document the contamination in your QC file trying to identify the tissue source;
2- train the embedding histotechs in the proper way of cleaning the embedding instruments and the forceps wells and document it also in the corresponding QC file;
3- save the slide marking the contaminating tissue fragment;
4- remove the contaminant and recut the block. Do not melt the block down, just remove the contaminant with a needle.
5- the slide to be filed with the case is the recut (step 4).
Probably other people handle this issue differently, but that is how I handled it
René J.

From: Roger Heyna <rheyna <@t> lumc.edu>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Wednesday, July 24, 2013 11:05 AM
Subject: [Histonet] Embedding Contamination

Hi Histonetters:

When a pathologist identifies extraneous tissue on a slide, and histology determines that the tissue contamination originates from embedding, what is your procedure for correcting the embedding? Do you remove the extraneous (or contamination) tissue from the block, recut the slide, and discard the original contaminated slide? Do you leave the extraneous tissue in the block and make a note in the report and/or on the slide? Do you keep track of these in a log somewhere?

Thanks in advance for your help.
Maywood, IL

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