[Histonet] Re: Carnoy solution
jaylundgren <@t> gmail.com
Sun Jul 21 14:42:49 CDT 2013
Whenever I am asked to make Carnoy's I reply, "Vroom-vroom!".
Jay A. Lundgren, M.S., HTL
On Fri, Jul 19, 2013 at 11:56 AM, Bob Richmond <rsrichmond <@t> gmail.com> wrote:
> Carnoy's fixative contains chloroform, and that's enough reason not to use
> it. In addition to its toxicity, chloroform dissolves methacrylate plastic,
> as I found out the hard way once when I tried to fix a specimen in Carnoy's
> fixative in a methacrylate container because I had nothing else.
> I've seen Carnoy's fixative used as a "disclosing fixative" for finding
> mesenteric lymph nodes in colectomy specimens. There's no excuse for this.
> There are a number of proprietary fixatives (such as Surgipath's O-Fix) for
> this purpose, or if you're allowed to brew your own, I use Davidson's
> fixative (3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde
> [NOT 10% NBF], 1 part glacial acetic acid.)
> Bob Richmond
> Samurai Pathologist
> Maryville TN
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