[Histonet] Re: Carnoy solution

[Jay Lundgren]

Whenever I am asked to make Carnoy's I reply, "Vroom-vroom!".

                                        Sincerely,

                                             Jay A. Lundgren, M.S., HTL
(ASCP)


On Fri, Jul 19, 2013 at 11:56 AM, Bob Richmond <rsrichmond <@t> gmail.com> wrote:

> Carnoy's fixative contains chloroform, and that's enough reason not to use
> it. In addition to its toxicity, chloroform dissolves methacrylate plastic,
> as I found out the hard way once when I tried to fix a specimen in Carnoy's
> fixative in a methacrylate container because I had nothing else.
>
> I've seen Carnoy's fixative used as a "disclosing fixative" for finding
> mesenteric lymph nodes in colectomy specimens. There's no excuse for this.
> There are a number of proprietary fixatives (such as Surgipath's O-Fix) for
> this purpose, or if you're allowed to brew your own, I use Davidson's
> fixative (3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde
> [NOT 10% NBF], 1 part glacial acetic acid.)
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>