[Histonet] RE: Histonet Digest, Vol 116, Issue 16
Suresch, Donna L.
donna_suresch <@t> merck.com
Wed Jul 17 12:34:22 CDT 2013
RE: Microtome Aligners
Maria,
Those microtome aligners will only be helpful in aligning all your microtomes to the same angle. They do not help you find the optimal angle.
Donna L. Suresch
Senior Imaging Research Scientist
Merck Research Laboratories
Department of Imaging - West Point Campus
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, July 17, 2013 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 116, Issue 16
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Today's Topics:
1. Sub-optimal Results (Amber McKenzie)
2. Searching for HHV-7 antibody (Lewis, Patrick)
3. Positions in SF area (Jennifer MacDonald)
4. RE: Sub-optimal Results (Sebree Linda A)
5. RE: Sub-optimal Results (joelle weaver)
6. RE: Competency for Anatomic and Clinical Pathology
(Michelle Lamphere)
7. microtome aligner (Maria Mejia)
8. RE: Competency for Anatomic and Clinical Pathology
(Morken, Timothy)
9. Re: microtome aligner (Grantham, Andrea L - (algranth))
10. RE: microtome aligner (Goins, Tresa)
11. Re: microtome aligner (Paula Sicurello)
12. RE: microtome aligner (joelle weaver)
13. Lab Equipment Repair in AZ?? (Vanessa Avalos)
14. My perfusion problem (Leila Etemadi)
----------------------------------------------------------------------
Message: 1
Date: Tue, 16 Jul 2013 19:24:50 +0000
From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] Sub-optimal Results
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6BB13C <@t> JERRY.Gia.com>
Content-Type: text/plain; charset="us-ascii"
Do you guys keep the QA form when a specimen is rejected and sent back to the source for correction? ANP. 11475
------------------------------
Message: 2
Date: Tue, 16 Jul 2013 20:02:45 +0000
From: "Lewis, Patrick" <patrick.lewis <@t> seattlechildrens.org>
Subject: [Histonet] Searching for HHV-7 antibody
To: "'Histonet <@t> lists.utsouthwestern.edu'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3903BE18914F4440834F0E620415FFCA25AC7AF1 <@t> PPWEXD01a.childrens.sea.kids>
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HI everyone,
Does anyone know where to get 5E1 mouse monoclonal antibody directed against HHV-7 (pp85 protein complex)
We tried two suppliers but their product has been discontinued and now we can not seem to find it anywhere.
Thanks
Patrick.
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Message: 3
Date: Tue, 16 Jul 2013 13:47:16 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: [Histonet] Positions in SF area
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF3C951EDB.47559B96-ON88257BAA.0071EBF7-88257BAA.0072054E <@t> mtsac.edu>
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I have a graduate that is looking for a Histotechnician position in the
Bay area. He is currently working for a dermatology lab. He would like
to relocate to the Bay area. If you know of any positions would you
please let me know. Thank you,
Jennifer MacDonald
Mt. San Antonio College
909-274-4884
jmacdonald <@t> mtsac.edu
------------------------------
Message: 4
Date: Tue, 16 Jul 2013 21:51:08 +0000
From: Sebree Linda A <LSebree <@t> uwhealth.org>
Subject: [Histonet] RE: Sub-optimal Results
To: 'Amber McKenzie' <amber.mckenzie <@t> gastrodocs.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77DD817201982748BC67D7960F2F76AF051CC3 <@t> UWHC-MBX12.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
We are required to keep all QA forms for 2 years.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Tuesday, July 16, 2013 2:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sub-optimal Results
Do you guys keep the QA form when a specimen is rejected and sent back to the source for correction? ANP. 11475
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------------------------------
Message: 5
Date: Tue, 16 Jul 2013 22:23:35 +0000
From: joelle weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] Sub-optimal Results
To: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT135-W513C3D7CF431FF843A14CD8600 <@t> phx.gbl>
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Yes absolutely.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: amber.mckenzie <@t> gastrodocs.net
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 16 Jul 2013 19:24:50 +0000
> Subject: [Histonet] Sub-optimal Results
>
> Do you guys keep the QA form when a specimen is rejected and sent back to the source for correction? ANP. 11475
>
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
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------------------------------
Message: 6
Date: Wed, 17 Jul 2013 13:10:02 +0000
From: Michelle Lamphere <MICHELLE.LAMPHERE <@t> childrens.com>
Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Cc: Elma Cortinas <ELMA.Cortinas <@t> childrens.com>
Message-ID:
<EEE916A21070714F918C63C127E620A310D4DBDF <@t> CMCPBEXMAIL03.Childrens.med>
Content-Type: text/plain; charset="iso-8859-1"
I think a few people might find this interesting....
I recently attended a class about Competency Assessments in the lab. The class was given by Ken Byrd (fairly certain that is how you spell his name), a Senior Inspector at CAP. When this particular question came up, I asked him to give examples of how the histology lab was supposed to use the 6 elements to assess competency. He informed the entire class that the competency assessment question with the six elements did not apply to the histology lab because histology did not report test results. It is the one question on the Gen Lab checklist that did not apply to ANP. Kinda shocking, I know.
It does not mean that we scrapped our entire competency program, we simply removed some of the six elements.
Michelle Lamphere
Senior Tech, Histology
Anatomic Pathology
Children's Medical Center
O: 214.456.2318 | Fax: 214.456.0779
E: michelle.lamphere <@t> childrens.com
1935 Medical District Drive | B1.06 ?| Dallas, Texas 75235
Message: 9
Date: Tue, 16 Jul 2013 07:14:08 -0400
From: "Histology" <histo <@t> pathlab.us>
Subject: [Histonet] Competency for Anatomic and Clinical Pathology at
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4 <@t> dc01.DominionPath.local>
Content-Type: text/plain; charset="us-ascii"
Hi,
I would love to see your competency spreadsheet for histology. We just finished our first CAP inspection and got a deficiency here. He said the direct observation was great, but that we need to have all 6 elements. I am having trouble trying to come up with a way to evaluate some of these and would love any help.
Thanks in advance,
Mehndi Helgren
Dominion Pathology Laboratories
733 Boush St.
Suite 200
Norfolk, VA 23510
757-664-7901
histo <@t> pathlab.us
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the intended recipient, any disclosure, copying, printing, or use of this information is
strictly prohibited and possibly a violation of federal or state law and regulations. If you
have received this information in error, please notify Children's Medical Center Dallas
immediately at 214-456-4444 or via e-mail at privacy <@t> childrens.com. Children's Medical
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------------------------------
Message: 7
Date: Wed, 17 Jul 2013 07:12:11 -0700
From: Maria Mejia <mbmphoto <@t> gmail.com>
Subject: [Histonet] microtome aligner
To: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <7814CEF8-9D00-4B49-BCB6-FEDD10559178 <@t> gmail.com>
Content-Type: text/plain; charset=iso-8859-1
Hello,
Can anyone provide any additional information regarding microtome aligners?
Do they work? I have never used or seen this particular tool, so which brand works
best? I know of only one company that sells a microtome aligner (universal type).
Any information any one can provide will be most appreciated.
Best
Maria Mejia
San Francisco, CA
------------------------------
Message: 8
Date: Wed, 17 Jul 2013 15:09:10 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology
To: "Michelle Lamphere" <MICHELLE.LAMPHERE <@t> childrens.com>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <761E2B5697F795489C8710BCC72141FF08704F <@t> ex07.net.ucsf.edu>
Content-Type: text/plain; charset=iso-8859-1
Yes, that is "interesting" considering that competencies are some of the primary documents that the Joint Commission inspectors ask for every time. And JC is under the same rules as CAP. So what is CAP's deal?
BTW, What was this class focused on? Not histology? Clincal lab, cytology? Curious.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere
Sent: Wednesday, July 17, 2013 6:10 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Cc: Elma Cortinas
Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology
I think a few people might find this interesting....
I recently attended a class about Competency Assessments in the lab. The class was given by Ken Byrd (fairly certain that is how you spell his name), a Senior Inspector at CAP. When this particular question came up, I asked him to give examples of how the histology lab was supposed to use the 6 elements to assess competency. He informed the entire class that the competency assessment question with the six elements did not apply to the histology lab because histology did not report test results. It is the one question on the Gen Lab checklist that did not apply to ANP. Kinda shocking, I know.
It does not mean that we scrapped our entire competency program, we simply removed some of the six elements.
Michelle Lamphere
Senior Tech, Histology
Anatomic Pathology
Children's Medical Center
O: 214.456.2318 | Fax: 214.456.0779
E: michelle.lamphere <@t> childrens.com
1935 Medical District Drive | B1.06 ?| Dallas, Texas 75235
Message: 9
Date: Tue, 16 Jul 2013 07:14:08 -0400
From: "Histology" <histo <@t> pathlab.us>
Subject: [Histonet] Competency for Anatomic and Clinical Pathology at
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4 <@t> dc01.DominionPath.local>
Content-Type: text/plain; charset="us-ascii"
Hi,
I would love to see your competency spreadsheet for histology. We just finished our first CAP inspection and got a deficiency here. He said the direct observation was great, but that we need to have all 6 elements. I am having trouble trying to come up with a way to evaluate some of these and would love any help.
Thanks in advance,
Mehndi Helgren
Dominion Pathology Laboratories
733 Boush St.
Suite 200
Norfolk, VA 23510
757-664-7901
histo <@t> pathlab.us
Please consider the environment before printing this e-mail.
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recipient, further disclosures are prohibited without proper authorization. If you are not
the intended recipient, any disclosure, copying, printing, or use of this information is
strictly prohibited and possibly a violation of federal or state law and regulations. If you
have received this information in error, please notify Children's Medical Center Dallas
immediately at 214-456-4444 or via e-mail at privacy <@t> childrens.com. Children's Medical
Center Dallas and its affiliates hereby claim all applicable privileges related to this
information.
_______________________________________________
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------------------------------
Message: 9
Date: Wed, 17 Jul 2013 15:14:16 +0000
From: "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
Subject: Re: [Histonet] microtome aligner
Cc: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <84CFE38D-CA15-4427-A370-D442ECE8CFE4 <@t> email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"
They do work. I saw one at NSH some years ago and came home and had the guys in our shop make one for me exactly like the one I saw and it cost next to nothing. We bought the little round level at home depot.
Andi
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724
algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415 Fax: 520.626.2097
------------------------------
Message: 10
Date: Wed, 17 Jul 2013 15:59:52 +0000
From: "Goins, Tresa" <TGoins <@t> mt.gov>
Subject: RE: [Histonet] microtome aligner
To: Maria Mejia <mbmphoto <@t> gmail.com>, HistoNet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FA81E534F40DEF44A70F0FBBCD49D2E403CDE425 <@t> doaisd5235.state.mt.ads>
Content-Type: text/plain; charset="us-ascii"
Yikes. There is a lot of "play" getting that bubble in the middle of the "universal" aligner that may translate to large variations in alignment each time the thing is used. The high cost is probably for the clamp, but if the bubble level is worth $1.50 the quality of the clamp doesn't matter.
Save your money and use a blank paraffin block.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Maria Mejia
Sent: Wednesday, July 17, 2013 8:12 AM
To: HistoNet
Subject: [Histonet] microtome aligner
Hello,
Can anyone provide any additional information regarding microtome aligners?
Do they work? I have never used or seen this particular tool, so which brand works best? I know of only one company that sells a microtome aligner (universal type).
Any information any one can provide will be most appreciated.
Best
Maria Mejia
San Francisco, CA
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Wed, 17 Jul 2013 12:13:07 -0400
From: Paula Sicurello <patpxs <@t> gmail.com>
Subject: Re: [Histonet] microtome aligner
To: "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
Cc: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAPSjddZ5cY2gEeSFJSsH9Fc6NN0Y7PDZA_65xeP7Bu6=K67dJQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I used an even cheaper version of the aligner. My microtome service
engineer suggested an "L level" or a "T" level that you can buy at Home
Depot. The L looks just like an L, it is actually a right angle measuring
device, used to make sure the corners or something are straight at 90
degrees.
You take the knife holder out and place one side of the L on the knife
holder place and the other against the block face.
Paula :-)
--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P: 919.684.2091
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On Wed, Jul 17, 2013 at 11:14 AM, Grantham, Andrea L - (algranth) <
algranth <@t> email.arizona.edu> wrote:
> They do work. I saw one at NSH some years ago and came home and had the
> guys in our shop make one for me exactly like the one I saw and it cost
> next to nothing. We bought the little round level at home depot.
>
> Andi
>
>
>
>
> Andrea Grantham, HT (ASCP)
> Senior Research Specialist
> University of Arizona
> Cellular and Molecular Medicine
> Histology Service Laboratory
> P.O.Box 245044
> Tucson, AZ 85724
>
> algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
> Tel: 520.626.4415 Fax: 520.626.2097
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 12
Date: Wed, 17 Jul 2013 16:34:05 +0000
From: joelle weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] microtome aligner
To: "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
Cc: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT135-W50295A5CD89F99F5F940A4D8610 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Yes you can certainly align the old fashioned way easy enough, but I find these are very handy and do save some time.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: algranth <@t> email.arizona.edu
> CC: histonet <@t> lists.utsouthwestern.edu
> Date: Wed, 17 Jul 2013 15:14:16 +0000
> Subject: Re: [Histonet] microtome aligner
>
> They do work. I saw one at NSH some years ago and came home and had the guys in our shop make one for me exactly like the one I saw and it cost next to nothing. We bought the little round level at home depot.
>
> Andi
>
>
>
>
> Andrea Grantham, HT (ASCP)
> Senior Research Specialist
> University of Arizona
> Cellular and Molecular Medicine
> Histology Service Laboratory
> P.O.Box 245044
> Tucson, AZ 85724
>
> algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
> Tel: 520.626.4415 Fax: 520.626.2097
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Wed, 17 Jul 2013 16:35:29 +0000
From: Vanessa Avalos <vavalos <@t> allergydermatology.com>
Subject: [Histonet] Lab Equipment Repair in AZ??
To: "histonet-request <@t> lists.utsouthwestern.edu"
<histonet-request <@t> lists.utsouthwestern.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6a98ea2a7359418fa1bcbb0d57a879b7 <@t> BY2PR07MB217.namprd07.prod.outlook.com>
Content-Type: text/plain; charset="us-ascii"
We recently found out that our equipment repair tech has retired. For our PM's and small repairs of our non contracted lab equipment we have used a local company for years. Sad to see him go. Does any one have any other local medical lab equipment techs/companies out in AZ (Phoenix) they would recommend?
I am not looking for reps to offer warranties, only recommendations at this time.
Thanks in advance!
V.Avalos
ADS, INC
Fax:602-277-2134
------------------------------
Message: 14
Date: Wed, 17 Jul 2013 16:39:31 +0000
From: Leila Etemadi <leila.etemadi <@t> med.lu.se>
Subject: [Histonet] My perfusion problem
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <52551199-3F4A-451B-A02E-0D6B06529AA6 <@t> med.lu.se>
Content-Type: text/plain; charset="utf-8"
Hi,
I am desperately looking for an answer to my problem in perfusion procedure.
What is my problem?
To explain that I thought first I may clear what is my usual routin.
I used to perfuse rat's brain through this procedure:
1- Anesthetise animal with sufficient amount of pentobarbital.
2- Clamp the vessel lying beside vertebra in the back, and then using a perfusion pump with an 17 or 18 gauge blunted needle inserted into the ascending aorta.
3- Washout blood with 0.9% salin ( room temperature), 150-200 ml, 76 rpm speed ( usually I wait to see the out put solution from the heart is not looking so red/ blood look like).
4- Switch to the 4% cold paraformaldehyde ( with buffer, 7.4 pH) ( on ice cold), 150-200 ml, 46 rpm speed. ( some times I can see the formalin dance but not always !).
5- after using this amount of PFA normally the neck part is quite stiff, then animal will be decapitated. I extract the brain right away, postfix in PFA over the night ( 4??c in the refrigerator).
6- Move to the sucrose 20%. When I saw brain is sinking in the solution ( which normally it happens in one and half day), I freeze the brain on dry ice quickly.
The results of this procedure is good enough histology after dissection with cryostat ( 12-20??m).
For my recent project I need brain and spinal cord both so basically I skip clamping the back
along vertebra to circulate solution through whole body ( I didn't change any other steps, so I do
exactly whatever I mentioned above except for spinal cord post fixation in PFA is about 2 hours).
After sectioning ( 16??m), I've got a proper tissue from spinal cord but when it comes to the
brain result is quite different !!, when I replaced my sections on the slide ( with plus charge),
suddenly a lot of hole began to shaped in the tissue which practically ruined the entire
piece..!!!,
First I had no clue for what I could see but then I noticed during post fixation procedure, when I
transfer brain from PFA solution to the sucrose, brain is sinking right away ( it is not
floating at all, as it suppose to do..) !!!!!
So I've run another perfusion procedure but this time when I reached to the washing out by cold
PFA I didn't decreased the pump speed ( as normally I decrease it from 76 rpm to 46 rpm,
this time I run whole procedure with the speed of 76 rpm), on the other hand I used more PFA
solution ( about 350 ml PFA). After extracting brain I noticed brain was looking dried up.
during post fixation and cryoprotection procedure (sucrose solution), sinking was looking normal,
but after sectioning of course brain tissue was destroyed again!!!
Now, I need your expert advices for my problem. I apologise for my naivety on this subject in advance.
Humbly appreciate for your great time and attention in advance. I severely looking forward to your help and valuable tips.
With the best,
Leila
Leila Etemadi
M.Sc., Ph.D Candidate
Neuronano Research Center (NRC)
Lund University, BMC F10
Sweden
Tel: +46-46-2221503<tel:+46-46-2221503>
E-mail: Leila.Etemadi <@t> med.lu.se<mailto:Leila.Etemadi <@t> med.lu.se>
Sent from my iPad
------------------------------
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