[Histonet] Bone decalcifying/processing problem

[Orla M Gallagher]

Dear Histonetters,

Has anyone ever seen large holes/vacuoles in the bone marrow of mouse
long bones after decalcification and processing to wax? We've had this
problem with a study which was fixed in 4% paraformaldehyde and
decalcified at 4 degrees C over a 14 day period according to a
routinely-used protocol involving changes of 15% EDTA/0.5% PFA in PBS
pH 8.0, rinsed in PBS to remove EDTA before processing to paraffin
wax. The marrow is filled with large holes, almost like balloons. I'm
happy to send images to anyone who might be able to help!

Best wishes,
Orla

--
**************************
Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bone Research
Department of Human Metabolism
D Floor Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX
UK

Website: http://mellanbycentre.dept.shef.ac.uk

Tel:         0044114-2713337 (office)
              0044114-2713174 (lab)
E-Mail:    o.m.gallagher <@t> sheffield.ac.uk


STOP: Do you really need to print this e-mail?

BE GREEN: Keep it on the screen.


Times Higher Education University of the Year



Data protection and confidentiality:

The information contained in this message or any appended documents
may be privileged and confidential and is intended for the exclusive
use of the addressee(s). If you are not the addressee, any disclosure,
reproduction, distributions, other dissemination or use of this
message is strictly prohibited and may be unlawful. If you receive
this correspondence in error please contact the sender immediately and
permanently delete/destroy what you have received.