[Histonet] How to remove 80% ethanol from bones before decalcifying in EDTA

Orla M Gallagher o.m.gallagher <@t> sheffield.ac.uk
Fri Jul 12 09:43:23 CDT 2013

Dear Histonetters,

I have some mouse bones samples which have been fixed in 70% ethanol
for a few weeks and stored in 80% ethanol for a week; now our
colleague would like to decalcify them in EDTA for wax embedding and
staining. What is the best way to remove the ethanol before transfer
to EDTA?


Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bone Research
Department of Human Metabolism
D Floor Medical School
University of Sheffield
Beech Hill Road
S10 2RX

Website: http://mellanbycentre.dept.shef.ac.uk

Tel:         0044114-2713337 (office)
              0044114-2713174 (lab)
E-Mail:    o.m.gallagher <@t> sheffield.ac.uk

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