[Histonet] How to remove 80% ethanol from bones before decalcifying in EDTA

[Orla M Gallagher]

Dear Histonetters,

I have some mouse bones samples which have been fixed in 70% ethanol
for a few weeks and stored in 80% ethanol for a week; now our
colleague would like to decalcify them in EDTA for wax embedding and
staining. What is the best way to remove the ethanol before transfer
to EDTA?

Thanks,
Orla

--
**************************
Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bone Research
Department of Human Metabolism
D Floor Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX
UK

Website: http://mellanbycentre.dept.shef.ac.uk

Tel:         0044114-2713337 (office)
              0044114-2713174 (lab)
E-Mail:    o.m.gallagher <@t> sheffield.ac.uk


STOP: Do you really need to print this e-mail?

BE GREEN: Keep it on the screen.


Times Higher Education University of the Year



Data protection and confidentiality:

The information contained in this message or any appended documents
may be privileged and confidential and is intended for the exclusive
use of the addressee(s). If you are not the addressee, any disclosure,
reproduction, distributions, other dissemination or use of this
message is strictly prohibited and may be unlawful. If you receive
this correspondence in error please contact the sender immediately and
permanently delete/destroy what you have received.