[Histonet] DAB enhancer

[Richard Cartun]

I don't know why anyone would want to use a DAB enhancer for slides prepared on Leica-Microsystems' Bond Max platforms.  The resulting immunoreactivity is exceptionally "Robust" in my experience when using their Bond Polymer Refine Detection kit.  Could this request be for a protein target expressed at low levels?  Is your hematoxylin too dark obscuring the immunoreactivity?
 
Richard


Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> Martha Ward-Pathology <mward <@t> wakehealth.edu> 7/11/2013 2:48 PM >>>
I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments.   Are many of you using this on the Bond, or any other staining platform and what are the pros and cons of its use?

Thanks!


Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mward <@t> wakehealth.edu  





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