[Histonet] Section cells on a plastic trans well membrane
Grantham, Andrea L - (algranth)
algranth <@t> email.arizona.edu
Wed Jul 10 09:40:20 CDT 2013
I do these frequently. The lab requesting the work usually does the fixing but I'm sure they do longer than 5 minutes. They then replace the fixative with 70% ETOH and bring the whole thing to the lab.
I use a tip of a scalpel blade to remove the whole membrane and I process it in a screen cassette or a bx cassette with small holes. I don't like to put anything on top of the membrane that might disturb the cells growing on it.
When embedding I cut the membrane circle in half and embed it standing up with the cut side down. I always use charged slides and have done H&E's and various special stains on the membranes and have never had one lift off. I usually let dry overnite and then put the slides in a drying oven with gentle heat for maybe 15 minutes before staining.
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
Tucson, AZ 85724
algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415 Fax: 520.626.2097
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