[Histonet] Question about sectioning brain tissue at 10-15 microns

[Tim Wheelock]

Hi Christina:

I use Paraplast to infiltrate the formalin fixed brain tissue, but I use 
Surgipath Embedding Medium from Leica (Catalogue number 3801320)  to 
embed the tissue.
(Surgipath does have about the same melting point as the Paraplast)
Even the 10 micron sections (for the Congo red stain) tend to curl, but 
I just guide them off of the disposable blade with a brush or a pick.

Hope this helps,

Tim

Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont MA
617-855-3592