[Histonet] Mouse brains

Davis, Cassie CDavis <@t> che-east.org
Thu Jan 17 12:10:27 CST 2013

      we used to cut human brains on a room temp water bath at a hospital I worked at many years ago. It might work for mouse brains too.

Cassie Davis

----- Original Message -----
From: "Michael J. Lyon, Ph.D." <lyonm <@t> upstate.edu>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 17, 2013 10:43:02 AM
Subject: [Histonet] Paraffin sections mouse brain

Hi everyone:

While I have sectioned a lot of different stuff, I have not done mouse brain
before and I am having considerable difficulty. ??The brains are fixed and
were embedded by our pathology department on their automated system. ??When
cutting, the paraffin cuts nicely but the brain tissue shreds as if the
infiltration was insufficient. ??I have re-embedded one, just melted and new
paraffin then vacuum oven for 2 hours. ??The results were the same. ??I don't
know if it would help to re-embed going back to EtOH. ??Also, from my distant
past I recall that when we were paraffin sectioning whole human larynges,
that the face of the block was soaked by placing a wet gauze pad on it. ??I
know this help but don't remember what the solution was on the pad. ??Any
help would be appreciated.




Michael J. Lyon, PhD

Otolaryngology Research Labs

SUNY Upstate Medical University

750 East Adams Street

Syracuse, NY 13210


Voice: 315-464-7253

Fax: ?? ?? 315-464-5572


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Message: 12
Date: Thu, 17 Jan 2013 11:21:40 -0600
From: Rita <1949rlh <@t> gmail.com>
Subject: [Histonet] Cpt code 88305
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4140C032-E482-45E2-A755-1545D0A66916 <@t> gmail.com>
Content-Type: text/plain;       charset=us-ascii

Sorry wrong code it is 88305
I need to know where to get information

Sent from my iPhone


Message: 13
Date: Thu, 17 Jan 2013 11:52:17 -0600
From: Glen Dawson <ihcman2010 <@t> hotmail.com>
Subject: [Histonet] MOHs Training
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU172-W30E142B8158A1B57093FFECC130 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

From: ihcman2010 <@t> hotmail.com
To: histonet-request <@t> lists.utsouthwestern.edu
Subject: FW: MOHs Training
Date: Thu, 17 Jan 2013 07:18:28 -0600


My lab is in the process of aiding one of our hospital's sites with MOHs and I'd like to find out what a MOHs lab might charge for training our techs

if there was such a service available?

What might that process be?  How long should it take to get a good histotech with no MOHs experience up and running?

What do other labs' training processes look like compared to ours?  Is there any place that trains outside techs for such a circumstance?

Do you have national contacts that may know someone who knows someone, etc.?

Any answers to any/all of the questions above would be an enormous help and would be greatly appreciated.

Thank-you in Advance,

Glen Dawson  BS, HT(ASCP), QIHC
Histology Technical Specialist
Mercy Health System
Janesville, WI


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