[Histonet] RE: Permanent Fluorescent Mounting Media

Paula Sicurello patpxs <@t> gmail.com
Tue Jan 15 12:49:43 CST 2013


When I was at MCV we used Crystal Mount for our frozen IF's (skin,
kidney, etc.)  You put a few drops on, place it in the oven for a
little bit (I do not recall the temperture of the oven) and you had a
nice hard cover over your sections.

I hope this helps.

Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091

On Tue, Jan 15, 2013 at 1:25 PM, James Watson <JWatson <@t> gnf.org> wrote:
> Has anyone used hardmount mounting medias for fluorescence in a high volume situation (up to 120 slides a day at times) when the slides are scanned?  We have tested BioCares, Thermofishers, and Vectashields.  We found that we liked the Thermo Immu-Mount best. Biocare Fluoro care antifade mountant worked, but with scanning we saw some stains were out of focus.  The VECTASHIELD HardSet Mounting Medium with DAPI gave us a higher percentage of fading and out of focus images.
> Our issue with the hardmount and scanning is that we see one edge of the slide in focus and the other out of focus frequently.  We are working on our mounting technique to see if this can be corrected by modifying our coverslipping technique.
> Any suggestions would be appreciated.  This does not make your fluorescence permanent since most fluorochromes fade with age.
> James Watson HT  ASCP
> GNF  Genomics Institute of the Novartis Research Foundation
> Tel    858-332-4647
> Fax   858-812-1915
> jwatson <@t> gnf.org
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Suresch, Donna L.
> Sent: Tuesday, January 15, 2013 10:08 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Permanent Fluorescent Mounting Media
> Hello Histonet,
> Anyone know of a non-aqueous permanent mounting media for use with fluorescent IHC?
> Donna L. Suresch
> Senior Imaging Research Scientist
> Merck Research Laboratories
> Department of Imaging - West Point Campus
> Mail Stop:  WP44K    Office: WP44-H129
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