[Histonet] Re: zebra fish experts......

[Hobbs, Carl]

Why are you fixing ?
Try unfixed frozen?
Or, why not Pwax embedded?
They cut well in Pwax.
Sure, you may have to "polish" the cut surface with tissue damped in water/20% alcohol to prevent cracking/curling just before each section is cut.

Or, after fixation, place in 30% sucrose until kidneys sink.
Store at 4C until you freeze ( if not immediately).
You will have to try several approaches until you find the correct one for your Lab.

Good luck.

Carl Hobbs
Histology and Imaging Manager
Wolfson CARD
School of Biomedical Sciences
Kings College London
Guys Campus
SE1 1UL
Tel: 020 78486813
Fax: 020 78486816
020 78486813