[Histonet] "harder" paraffin

Jack Ratliff ratliffjack <@t> hotmail.com
Fri Jan 11 11:45:53 CST 2013

Hello Betsy!
I use Leica's EM400 for my paraffin embedding needs and it does extremely well for all of my decalcified bone work. Of course I mostly work with undemineralized bone, biomaterials and medical device implants so I pretty routinely utilize acrylic resins (MMA) for most of my work. With that said, if you need any help or assistance should you need to go the route of plastic embedding, feel free to contact me.
One quick note, I also have access to a non-contact laser (laser microtome) that can cut thin micron linear or 3D sections of fresh or resin embedded tissues. If you are interested, I would love to see if this technology could help service your needs if you have some spare tissue samples that you could send over to me.
Best Regards,

Jack L. RatliffOwner/Histologist - Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology317-281-1975jratliff <@t> ratliffhistology.com

From: BMolinari <@t> texasheart.org
To: Histonet <@t> lists.utsouthwestern.edu
Date: Fri, 11 Jan 2013 17:31:02 +0000
Subject: [Histonet] "harder" paraffin

Hi Histonetters,
Good Friday to you all. Do you know if there is a “harder” paraffin  that may offer more support to the tissue while cutting? I currently use Paraplast Plus and love it, but now I am cutting some polymer material and there is some tearing and was thinking that maybe a different paraffin would help. I am trying to avoid embedding in plastic.
Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
1101 Bates Street
Houston,TX 77030
832-355-6524 (lab)
832-355-6812 (fax)
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Betsy Molinari
Senior Histology Research Technician
832-355-6524 | BMolinari <@t> texasheart.org<mailto:BMolinari <@t> texasheart.org>
6770 Bertner Ave. MC 1-283 | Houston, TX 77030
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