[Histonet] tricks about antibody generated in human on human tissue for IHC

[Morken, Timothy]

Carole, Look at Golden Bridge International Labs. They have a human-on-human detection system.

http://gbi-inc.com/

I've worked with them before and they have excellent products. They also supply some products to other vendors that are rebadged.


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of carole.chotard <@t> fr.netgrs.com
Sent: Friday, January 11, 2013 2:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tricks about antibody generated in human on human tissue for IHC

Dear all,

 

I work in an histopathology department and we intend to do immunohistochemistry with an antibody generated in human on human tissues. So far, we don't have any experience with that. So I was wondering if somebody would have some feedback to prevent strong background generated by this human antibody on human tissue. We are planning in a first place to decrease the concentration of the secondary antibody Donkey <@t> human which is recommended at 1/200 by Jackson, using it at 1/500 for example.

 

But if anybody would have some experience with this kind of experiments, their tricks would be more than welcome.

 

Best Regards,

 

Carole Chotard

 

 

Carole Chotard, Ph.D.

Pharmacology Research Scientist

Molecular Pharmacology and Pathophysiology Department

IDRS

125 Chemin de Ronde

78290 Croissy-sur-Seine

France

Phone : +33 1 55 72 29 48

Fax :       +33 1 55 72 25 96

carole.chotard <@t> fr.netgrs.com

 

 

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