[Histonet] Xylene free processing

Rene J Buesa rjbuesa <@t> yahoo.com
Thu Jan 10 14:10:58 CST 2013

Not even to clean the tissue processor you will need xylene if you prepare a 5% solution of liquid dish washer soap in isopropyl alcohol. Place this solution in the cleaning "used xylene" container and clean the tissue processor with it.
René J.

From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
To: histonet <histonet <@t> lists.utsouthwestern.edu> 
Sent: Thursday, January 10, 2013 1:13 PM
Subject: [Histonet] Xylene free processing


We have been processing xylene free for a couple of years in ThermoFisher Excelsior processors.  The tissue goes directly from isopropyl alcohol to paraffin.  It works well for us.  We sought out a xylene free protocol to reduce the amount of xylene in the lab.

We do need to use xylene in the cleaning cycle but it's only a gallon.

Good luck!


Erin Martin, Histology Supervisor
UCSF  Dermatopathology Service

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