[Histonet] her 2 neu, CISH vs FISH

[Cynthia Pyse]

Good afternoon histonetters

We are currently investigating bringing Her 2 Neu CISH staining into our
lab. FISH staining has too many logistics nightmares for me to consider, not
to mention the cost of purchasing digital scanning equipment. Our
pathologist are off site in 5 different hospitals.

 

Does anyone know if there is a sensitivity issue between fluoresce and
chromogen staining?  Are oncologists receptive to having the Her 2 Neu
confirmed by chromogenic ISH as opposed to fluoresce ISH? 

 

Anyone performing Her 2 Neu by CISH, what, if any, issues have you
encountered. 

 

Any help and information would be appreciated. Thanks in advance.

Cindy

 

Cindy Pyse, CLT, HT (ASCP)

Laboratory Manager

X-Cell Laboratories

20 Northpointe Parkway Suite 100

Amherst, NY 14228

716-250-9235 etx. 232

e-mail cpyse <@t> x-celllab.com