[Histonet] Xylene Free Processing?

[Finley, Sue [PH]]

We use xylene free processing + microwave
Formalin, 100% ethanol, isopropanol (clearant) and paraffin and have been doing so for 4 years

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: January 9, 2013 12:16 PM
To: Jones, Laura; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene Free Processing?

The Xpress microwave tx processors are xylene free - perhaps they have
one.

I am pretty certain there still needs to be something that is misable
with alcohol and paraffin, such as mineral oil. I believe this can be
added to one or two of the paraffin steps, but I am just venturing a
guess. Most processors would still require a xylene (or substitute)
cleaning cycle to remove paraffin. 

I will be following this thread with interest - Bill

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.

Cultivate it in PRAYER!

 






-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jones,
Laura
Sent: Wednesday, January 09, 2013 1:38 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Xylene Free Processing?

I would appreciate hearing all of your expert opinions on processing
tissue without the use of xylene or any type of xylene substitute.  Our
processor was down recently, and a friendly local lab processed our
tissue this way for us.  Of course, the Pathologists loved it.  The
process was formalin, followed by graduated percentages of only
isopropyl alcohol, and then paraffin.

What are your feelings concerning immunos?  Overall cost?  Any other
thoughts?

Thanks in advance!

________________________________

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