[Histonet] Glyco-Fixx

Mary Benoit mbplab <@t> yahoo.com
Wed Jan 2 13:47:49 CST 2013

Need help solving a fixation/processing problem.  We are a regional reference Pathology Lab and we recently began receiving GI biosies in Glyco-fix from Thermo Scientific.  Our standard processing for small biopsies is formalin 2 changes for 1hour and 1.5 hours; 70% for 15 minutes; 95% 2 changes 15 min each; 100% 3 changes 30 min each; xylene 2 changes 30 min each and paraffin 3 changes 30 min each.  Theis has worked great for years with my specimens received in formalin.  Now the biopsies received in glycol-fix have no nuclear detail, look "muddy" and I am not sure what to do or what to change in my protocol.  The pathologists are speaking with the clinicians, but it isa P.R. thing and this must be handled the right way.  I know the fixative is mostly alcohol blend with a little glyoxal, but even post fixing in 10%NBF is not helping.  Suggestions please!
Mary F Benoit
mbplab <@t> yahoo.com
The Pathology Laboratory
Lake Charles, LA

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