[Histonet] sections falling off

[Megha Kumar]

Hi Histonetters !
Can anyone suggest how to prevent  adult mouse skin sections from falling
off during in situ hybridization? How to make sections adhere better to
slides? I am using poly-lysine coated slides for 5-10 micron thick skin
sections. The tissue is fixed in 4% PFA and embedded in paraffin.
Please help !
thanks
megha


-- 
Megha Kumar, Ph.D.
Post-doctoral Research Scholar
Sen-Bandyopadhyay Lab
Department of Biological Sciences and Bioengineering
Indian Institute of Technology Kanpur
Kanpur, 208016
India

phone - 9695830033

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