[Histonet] Re: zebrafish and IHC

[Teri Johnson]

The differences might be due to decalcification and/or differences in fixation.  I presume you are decalcifying the fish? Are you using EDTA or a formic acid decalcifier? Are they fixed in 10%NBF (or equivalent) for roughly the same amount of time as your human samples? Or are you using a different fixative altogether.  The protocol might need to be re-optimized for your fish samples based on those issues.

Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752