[Histonet] Rodent eye paraffin microtomy

[Truscott, Tom]

Hi Jackie, I haven't done a lot of eyes, but mainly sheep lenses. I seemed to have more trouble with crushing artifact when trimming in- so I tried not to trim too close and do the closer trimming on the microtome- I also trimmed before they got too hard from fixing. Tom T

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra
Sent: Thursday, December 12, 2013 9:13 AM
To: 'b427297 <@t> aol.com'; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Rodent eye paraffin microtomy

Let the eye soak in ice water before cutting for a very long time.

Bea

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of b427297 <@t> aol.com
Sent: Thursday, December 12, 2013 8:48 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Rodent eye paraffin microtomy


I have a study coming up where obtaining perfect lens histology is critical.  We do a pretty good job routinely, but since the lens is of interest, I would appreciate any tricks and techniques that can help us improve our paraffin lens histology within intact rodent eyes.
Thanks,
Jackie O'
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