[Histonet] CD4 and CD8 mouse tumor IHC, thanks..

[Erickson, Jamie E]

Thank you to those that sent me suggestion on my CD4 problem. I have recently discovered the problem.
The tumors are mouse tumors but they are injected in-vivo with a test antibody that is Rat anti-mouse, specifically Isotype (Rat IgG2a). Hence this is why my secondary Rabbit anti-rat stained so much..

Next I will try to block the Rat in-vivo injected antibody with a goat anti-Rat secondary, as a  block prior to staining with CD4.
Or I could precomplex the CD4 with the Rabbit Anti-Rat and bind excess Anti-Rat with Rat Serum.

Any thoughts or suggestion on this as always would be welcomed...

Thanks again..

Jamie

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