AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde

[Wineman, Terra]

I would suggest a different protocol if the tissue will not be processed for a while.  I would say a week in 10%NBF and then transfer the bones to an EDTA decal solution.  The bones will decal slowly without the affects of the formic acid.  I am in research and this is what we do with our bones.

Terra Wineman, HTL (ASCP)CM
Research Biologist
636-926-7476 phone
terra.wineman <@t> novusint.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pruegg <@t> ihctech.net
Sent: Thursday, December 05, 2013 2:50 PM
To: gu.lang <@t> gmx.at; 'Orla M Gallagher'
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde

i would think u are correct in advising formic acid decal and then processing into paraffin for the best protection of the trap enzyme, immunoreactivity, etc.  A couple of weeks in formalin should be fine.  Paraformaldehyde show be the same as formalin.  I do know a way to restore the enzyme activity for TRAP that may have been lost so if u need that let me know.
 
--------- Original Message --------- Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Date: 12/5/13 11:42 am
To: "'Orla M Gallagher'" <o.m.gallagher <@t> sheffield.ac.uk>
Cc: histonet <@t> lists.utsouthwestern.edu

Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous  solution of formaldehyd. 
 So the main characteristics are the same.
 
 Gudrun Lang
 
 -----Urspr&uuml;ngliche Nachricht-----
 Von: histonet-bounces <@t> lists.utsouthwestern.edu
 [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Orla M  Gallagher
 Gesendet: Donnerstag, 05. Dezember 2013 19:31
 An: histonet <@t> lists.utsouthwestern.edu
 Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4%  paraformaldehyde
 
 Dear Histonetters,
 
 What is your opinion on storing bone samples long-term (more than a couple  of weeks) in 10% formalin? As I was taught, best practice has always been to  fix only as long as necessary, depending on the size of the sample, then  decalcify and process to wax, and I always stress this to everyone I advise.
 
 However, research colleagues sometimes wish to do histology on bone samples  that have been stored for months ..or even years! As the formalin pH becomes  more acidic, there is formalin pigment and the immunoreactivity and TRAP  enzyme activity is diminished or destroyed during long fixation, is there  any way of minimising this e.g. has anyone tried regularly replacing the old  formalin with fresh buffered formalin, or storing formalin-fixed bones in  any other medium? I'm also interested in how best to fix in 4%  paraformaldehyde and whether the problems are the same with long-term  storage.
 
 Thanks for your comments.
 
 All the best,
 Orla
 
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 Ms. Orla Gallagher
 Bone Analysis Laboratory
 Mellanby Centre for Bone Research
 Department of Human Metabolism
 D Floor Medical School
 University of Sheffield
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