[Histonet] RE: IHC Tissue

Sebree Linda A LSebree <@t> uwhealth.org
Wed Aug 28 06:39:07 CDT 2013


Hi Colleen,

We are just wrestling with this very issue and have decided to use:

	Normal colon, i.e. staining present with all four antibodies
	Colon carcinoma without the mutation, i.e. staining present with all four antibodies
	Colon carcinoma(s) with the mutation for each antibody, i.e. no staining present for each of the four antibodies, probably employing two different specimens

I'll be interested in your other responses since our plan is not yet set in stone.

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Herring, Colleen
Sent: Wednesday, August 28, 2013 6:14 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Tissue

Good morning,

   I would like to get some input on the type of tissue everyone is using for their IHC MMR protocol. I would like to use just one block for all four protocols if possible. Any suggestions.

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