[Histonet] Bone/cartilage/epithelial tissue stain

[Hobbs, Carl]

A H&E will allow you to identify those three tissue types, in  normal tissues, of most organisms ( certainly 
in avian embryos, imho), IF you  know your Histology.

There is no stain/demonstration technique that will substitute for this knowledge, imho.
Sure, a "special stain" will help to confirm the tissue type to a high degree.
I usually start with staining the section with Alcian blue pH3, then place in Hx ( I use Gill's 2....sure, they state you must use Iron Hx...not necessary, for me) for 10 mins.
Then place in Van Gieson's solution until what I know is collagen type I is red.

I hope this adds to previous excellent posts.
Best regards,

Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 
020 7848 6813