[Histonet] Bone/cartilage/epithelial tissue stain

["E. Wayne Johnson 朱稳森"]

At one time I did a lot of work on cartilage and growth plate
and used Toluidine Blue and Fast Green.  T-blue (buffered appropriately) 
stains the
proteoglycan a lovely metachromatic blue and the bone and most 
everything else
is green.  The nuclei of cells are also green since we used no nuclear 
stain, but
that was not a problem for our work.

The cartilage is in stark contrast to the bone because of the high 
carbohydrate
(glycosaminoglycan/proteoglycan) content of cartilage.

On 3:59, Rui TAHARA wrote:
> Hi,
> Would anyone suggest me what staining is
> best to color differentiate between cartilage and bone and epithelial tissues
> in avian embryos?
>
> I have been trying Mallory Trichrome for
> embryos but recently I was suggested that Mallory Trichrome stains cartilage differently
> in embryos compared to adult samples since Aniline blue stains fiber that may
> not develop in early embryos. There is some protocol that modified the Mallory Trichrome
> that could be applied to embryos. However, the resulting colors of all tissues
> look all purple-ish and difficult to tell the cartilage from the weak blue
> stain from aniline blue.
>
> Currently I am thinking to try out Alcian
> blue/Hematoxylin and Eosin stain (Ehrlich’s hematoxylin). The purpose of the
> staining is to look at interaction between ossification and epithelial development
> so I think alcian blue for staining cartilage works but I am wondering if there
> is any other staining combination with alcian blue exist for visualizing bone
> and epithelial tissue (e,g. alcian blue/alizarine red with other staining?).
>