[Histonet] Bone/cartilage/epithelial tissue stain

[Elizabeth Chlipala]

Rui

There are two proteoglycan stains they you may want to try.  The two stains that come to mind are Safranin O and Toluidine blue, these will stain for proteoglycan and therefore the cartilage will stain positive.  Bone will not stain much.  You could try a Movats that might be helpful.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rui TAHARA
Sent: Friday, August 23, 2013 1:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bone/cartilage/epithelial tissue stain



Hi,
Would anyone suggest me what staining is best to color differentiate between cartilage and bone and epithelial tissues in avian embryos?

I have been trying Mallory Trichrome for embryos but recently I was suggested that Mallory Trichrome stains cartilage differently in embryos compared to adult samples since Aniline blue stains fiber that may not develop in early embryos. There is some protocol that modified the Mallory Trichrome that could be applied to embryos. However, the resulting colors of all tissues look all purple-ish and difficult to tell the cartilage from the weak blue stain from aniline blue.

Currently I am thinking to try out Alcian blue/Hematoxylin and Eosin stain (Ehrlich's hematoxylin). The purpose of the staining is to look at interaction between ossification and epithelial development so I think alcian blue for staining cartilage works but I am wondering if there is any other staining combination with alcian blue exist for visualizing bone and epithelial tissue (e,g. alcian blue/alizarine red with other staining?).



Any suggestion would be appreciated!

Rui TAHARA
PhD Candidate
Biology Department
McGill University