[Histonet] Xylene Substitutes

[Dingersoll <@t> aplaboratories.com]

   Donna S. Ing
   Laboratory Manager

   A P Laboratories, LLC
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-------- Original M   
Subject: Re: [Histonet] Xylene Substitutes
   
From   
Date: Wed, August 21, 2013 12:06 pm
   
To:    "Yaskovich,    Ruth   skovich <@t> dir.nidcr.nih.gov>,
   
"'[4]Histonet <@t> lists.utsouthwestern.edu'"
   
<Histonet <@t> lists.utsouthwest   

   
Ruth:
   
I all reality none is good. B   as  dangerous  or more than xylene, and    standard.
   
Any  one  requires  modifications   dewax  adequately and all constitute a proce   cannot de recycled.
   
Please go to [5]http://www.histosearch.com/rene.html<   about xylene substitution.
   

   
The optimal solu   
1- dehydrate with 2-propanol (o   
2-  do  not  use  any  ante-medium  and     paraffin wax or ideally to mineral oil:IPA at    
3- infiltrate as usual
   
4-  dewax  sections with 2%aq. sol. of   for 1 min twice
   
5- stain as usual
   
   for 5 minut   
7- coverslip as usual.
   
As  you  can  see  xylene is nowhere to b   that produce optimal quality slides.
   
Try it!   
René J.
   

   

   
________________________________
   
From: "Yaskovich, Ruth A (NIH/NIDCR) [E]"
   <[6]ryaskovich <@t> dir.nidcr.nih.gov>
   
To:     "'[7]Histonet <@t> lists.utsouthwes   tonet <@t> lists.utsouthwestern.edu>
   
Sent: Wednesday, August 21, 2   
Subject: [Histonet] Xylene Substitutes
   

   

      and safes   
Ruth Yaskovich
   
N.I.H. N.I.D.C.R.
   
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References

   1. ="mailto:dingersoll <@t> aplaboratories.com"
   2. 3D"mailto:rjbuesa <@t> yahoo.com"
   3. 3D"mailto:ryaskovich <@t> dir.nidcr.nih.gov"
   4. 3D"mailto:Histonet <@t> lists   5. file://localhost/tmp/3D"   6. 3D"mailto:ryaskov   7. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
   8. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
   9. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
  10. 3D"http://lists.utsouthwestern.edu/mailma  11. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
  12. 3D"http://lists.utsouthw=/