[Histonet] Xylene Substitutes
Dingersoll <@t> aplaboratories.com
Dingersoll <@t> aplaboratories.com
Wed Aug 21 11:20:32 CDT 2013
Donna S. Ing
Laboratory Manager
A P Laboratories, LLC
2090 Executive Hall Rd S Charleston 843-300- 843-300- [1]dingersoll <@t> aplaboratories.com The contents of this message, together intended only for the use of the person(s) to whi addressed and may contain confidential and/or privileged inform ation. Further, any medical information herein is confidential and
protecte review, copy, dis information. If you are not the advise the sender and delete this message a distribution, or copying of this message, or any at prohibited.
-------- Original M
Subject: Re: [Histonet] Xylene Substitutes
From
Date: Wed, August 21, 2013 12:06 pm
To: "Yaskovich, Ruth skovich <@t> dir.nidcr.nih.gov>,
"'[4]Histonet <@t> lists.utsouthwestern.edu'"
<Histonet <@t> lists.utsouthwest
Ruth:
I all reality none is good. B as dangerous or more than xylene, and standard.
Any one requires modifications dewax adequately and all constitute a proce cannot de recycled.
Please go to [5]http://www.histosearch.com/rene.html< about xylene substitution.
The optimal solu
1- dehydrate with 2-propanol (o
2- do not use any ante-medium and paraffin wax or ideally to mineral oil:IPA at
3- infiltrate as usual
4- dewax sections with 2%aq. sol. of for 1 min twice
5- stain as usual
for 5 minut
7- coverslip as usual.
As you can see xylene is nowhere to b that produce optimal quality slides.
Try it!
René J.
________________________________
From: "Yaskovich, Ruth A (NIH/NIDCR) [E]"
<[6]ryaskovich <@t> dir.nidcr.nih.gov>
To: "'[7]Histonet <@t> lists.utsouthwes tonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, August 21, 2
Subject: [Histonet] Xylene Substitutes
and safes
Ruth Yaskovich
N.I.H. N.I.D.C.R.
___
Histonet mailing list
[9]Histonet <@t> lists.uts
[10]http://lists.utsouthwestern.edu/mailman/listinfo/histo
_______________________________________________
Histone
[11]H
[12]http://lists.utsouthwestern.edu/mailm
References
1. ="mailto:dingersoll <@t> aplaboratories.com"
2. 3D"mailto:rjbuesa <@t> yahoo.com"
3. 3D"mailto:ryaskovich <@t> dir.nidcr.nih.gov"
4. 3D"mailto:Histonet <@t> lists 5. file://localhost/tmp/3D" 6. 3D"mailto:ryaskov 7. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
8. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
9. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
10. 3D"http://lists.utsouthwestern.edu/mailma 11. 3D"mailto:Histonet <@t> lists.utsouthwestern.edu"
12. 3D"http://lists.utsouthw=/
More information about the Histonet
mailing list