[Histonet] Positive tissue controls for MSI antibody panel

[Tony Auge]

Ok, thanks anyway. I tried Cell Marque, they just have normal colon to use
for control tissue and they are $20 a slide. I am looking for tissue that
has microsatilite instability for MSH-2 and MSH-6.


On Mon, Aug 19, 2013 at 10:55 AM, Sebree Linda A <LSebree <@t> uwhealth.org>wrote:

> Question everyone:
>
> What are you all using for your positive tissue control when running the
> MSI antibody panels, i.e. MLH-1, MSH-2, MSH-6, PMS-2?  We're contemplating
> using normal colon that all four antibodies should stain but do we also
> need to show that colon having the mutation does not stain for each
> antibody as well to show there are no false positive results?
>
> The former would be the easy way out but I'm willing to construct a
> multi-tissue block containing normal colon and colon with the mutation(s).
>
> Thanks for all your responses,
>
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
>
>
>
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-- 

Tony Auge HTL (ASCP) QIHC
Cell: (651) 373-4768
Email: tony.auge <@t> gmail.com