[Histonet] Cautery-Like Artefact

[joelle weaver]

Drying is always a problem, with conventional or MW processing.  So I agree rule that out first.  




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: tony.henwood <@t> health.nsw.gov.au
> To: rheyna <@t> lumc.edu; bbrinegarhtl <@t> gmail.com
> Date: Tue, 20 Aug 2013 01:35:20 +0000
> Subject: RE: [Histonet] Cautery-Like Artefact
> CC: histonet <@t> lists.utsouthwestern.edu
> 
> In this case I would also look at fixation time - increase it as much as possible.
> 
> Regards 
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
> Laboratory Manager & Senior Scientist 
> Tel: 612 9845 3306 
> Fax: 612 9845 3318 
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roger Heyna
> Sent: Tuesday, 20 August 2013 7:25 AM
> To: Beth Brinegar
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Cautery-Like Artefact
> 
> Most of the derms are processed on Milestone's Pathos microwave processor. The processing program is 5 hours long.
>  
> Roger
> 
> >>> Beth Brinegar <bbrinegarhtl <@t> gmail.com> 8/19/2013 4:04 PM >>>
> How long is your overnight processing run? Are these specimens being processed on an eight hour run, a four hour run, or something like a rapid run?
> 
> On Monday, August 19, 2013, Roger Heyna wrote:
> 
> 
> We are experiencing a staining artefact on our H&E's, most commonly seen on skin specimens, but also observed on other specimen types as well.
> 
> Within the dermis and subcutaneous regions of the skin specimens, there are portions of what should be collagen that appear homogenized and stained with hematoxylin, instead of the usual eosin staining. It's often on the edge of the section and varies in size between different specimens. The pathologist is normally able to read around it, but there have been cases that were made more difficult to diagnosis by this artefact.
> 
> Our pathologists believe this artefact resembles cautery artefact, but the dermatologists insist they are not using cautery during collection.
> 
> We have a large derm service, and most of our derm specimens fix overnight. We have both microwave and conventional processing, and the "artefact" specimens have been processed on both. All of these specimens have grossing ink on them.
> 
> Any thoughts on what could be causing this? If it's not cautery, does it seem like a fixation issue? Has anyone seen grossing ink affect processing?
> 
> Thanks ahead of time for your help.
> Roger
> 
> 
> --
> Beth Brinegar HTL(ASCP)
> Anatomic Pathology Supervisor
> Mercy Medical Center
> Cedar Rapids, IA 52403
> 
> 
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