[Histonet] processing eyes

[b427297 <@t> aol.com]

We transfer eyes out of Mod Davidsons after 24 hours and process with the other soft tissues in formalin- why transfer to alcohol? We have beautiful  eye sections.
Jackie O'

Sent from my iPhone

On Aug 8, 2013, at 3:12 PM, Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com> wrote:

> Patsy, 
> 
> 18-24 hours in Davidson's followed by 70% ethanol prior to processing. Some people also store the eyes in 10%NBF. I will attach a schedule for you off line. 
> 
> Bea
> 
> Beatrice DeBrosse-Serra HT(ASCP)QIHC
> Isis Pharmaceuticals
> Antisense Drug Discovery
> 2855 Gazelle Ct.
> Carlsbad, CA 92010
> 760-603-2371
> 
> 
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pruegg <@t> ihctech.net
> Sent: Thursday, August 08, 2013 1:06 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] processing eyes
> 
> 
> Can someone direct me to a protocol for processing eyes which have been injected with Davidson's fixative and are now in 10% NBF.  We had an eye expert at the U for years I was going to send my techs to but apparently she has finally retired because we cannot get a hold of her.  Where are you Mary Jo?
> 
> 
> We need insight on how to process non-human primate eyes? They have been injected with Davidson's fixative and are now in 10%NBF. We need a specific processing schedule, embedding and sectioning advice.
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> Thank you,
> 
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> 
> Patsy
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> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 
> 40864 E. Arkansas Ave
> 
> Bennett, CO 80102
> 
> H 303-644-4538
> 
> C 720-281-5406
> 
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