[Histonet] processing eyes

[Bea DeBrosse-Serra]

Patsy, 

18-24 hours in Davidson's followed by 70% ethanol prior to processing. Some people also store the eyes in 10%NBF. I will attach a schedule for you off line. 

Bea

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pruegg <@t> ihctech.net
Sent: Thursday, August 08, 2013 1:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing eyes


Can someone direct me to a protocol for processing eyes which have been injected with Davidson's fixative and are now in 10% NBF.  We had an eye expert at the U for years I was going to send my techs to but apparently she has finally retired because we cannot get a hold of her.  Where are you Mary Jo?


We need insight on how to process non-human primate eyes? They have been injected with Davidson's fixative and are now in 10%NBF. We need a specific processing schedule, embedding and sectioning advice.

 

Thank you,

 

Patsy


 


 

 

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting

40864 E. Arkansas Ave

Bennett, CO 80102

H 303-644-4538

C 720-281-5406

 <mailto:pruegghm <@t> hotmail.com> pruegghm <@t> hotmail.com 

 <mailto:rueggihcconsultingpr <@t> outlook.com> rueggihcconsultingpr <@t> outlook.com

 

 

 

 


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