[Histonet] Invitrogen A6455 rabbit anti-GFP dilution for

[Hobbs, Carl]

 IF using Alexa - conjugated secondaries ( indirect IF) should give you the same results as a std  stABCpx-DAB detection, for any given dilution of  primary Ab.
However, if you are using enhanced DAB.....sure, the primary Ab will have to be less dilute when visualising with an  indirect IF method.
So, do one run with your primary at 1/5K, 1/10K, 1/20K ( on three sequential sections, of course) ...detecting with, for eg, Alexa anti Rabbit 594 at 1/1K.

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 
020 7848 6813