[Histonet] Portable fans in the lab

[Sanders, Jeanine (CDC/OID/NCEZID)]

We put freshly cut slides on the stainer regularly and we use charged slides exclusively. I simply take each slide and give each a quick sharp flick to snap the excess water out from under the section as I place them in the staining rack and then load on the stainer.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Victoria Baker
Sent: Sunday, August 04, 2013 12:32 PM
To: Rene J Buesa
Cc: histonet <@t> lists.utsouthwestern.edu; Jeanne Clark
Subject: Re: [Histonet] Portable fans in the lab

Hi
Rene is correct about standing them up right to drain, but if you are using charged slides water gets trapped underneath the sections because of the + charge of the slide itself.  If you see a bubble on the last section or around  sections on a ribbon you can release the water by just pin pricking them on the edge with a needle point or the tip of a fine tipped forceps. Tap the section and the water will drain away from the tissue.
Joelle is on target with use of a fan if your lab has an increased or high humidity level as this will also affect the water retention on a slide.






On Sun, Aug 4, 2013 at 11:50 AM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> In my experience the best procedure is to place the slides with the 
> sections just "fished" from the water bath in a vertical position on 
> the short edge (1 plg).
> By doing so the water that may be under the section will come out and 
> after about 5 minutes the slides can be placed in the rack and either 
> go to the oven to dry before manual staining or directly to the 
> autostainer heating station.
> If you blow air over a section that is in horizontal position any 
> water trapped under the section will remain there and can cause the 
> artifact known as "nuclear bubbling".
> René J.
>
>
> ________________________________
> From: Jeanne Clark <paintedsplashes <@t> yahoo.com>
> To: "histonet <@t> lists.utsouthwestern.edu" 
> <histonet <@t> lists.utsouthwestern.edu
> >
> Sent: Saturday, August 3, 2013 5:34 PM
> Subject: [Histonet] Portable fans in the lab
>
>
> Anyone using portable fans in Histology to facilitae drying of parafin 
> sections on slides prior to drying in oven?
>
> Jeanne
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