[Histonet] Portable fans in the lab

[Victoria Baker]

Hi
Rene is correct about standing them up right to drain, but if you are using
charged slides water gets trapped underneath the sections because of the +
charge of the slide itself.  If you see a bubble on the last section or
around  sections on a ribbon you can release the water by just pin pricking
them on the edge with a needle point or the tip of a fine
tipped forceps. Tap the section and the water will drain away from the
tissue.
Joelle is on target with use of a fan if your lab has an increased or high
humidity level as this will also affect the water retention on a slide.






On Sun, Aug 4, 2013 at 11:50 AM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> In my experience the best procedure is to place the slides with the
> sections just "fished" from the water bath in a vertical position on the
> short edge (1 plg).
> By doing so the water that may be under the section will come out and
> after about 5 minutes the slides can be placed in the rack and either go to
> the oven to dry before manual staining or directly to the autostainer
> heating station.
> If you blow air over a section that is in horizontal position any water
> trapped under the section will remain there and can cause the artifact
> known as "nuclear bubbling".
> René J.
>
>
> ________________________________
> From: Jeanne Clark <paintedsplashes <@t> yahoo.com>
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu
> >
> Sent: Saturday, August 3, 2013 5:34 PM
> Subject: [Histonet] Portable fans in the lab
>
>
> Anyone using portable fans in Histology to facilitae drying of parafin
> sections on slides prior to drying in oven?
>
> Jeanne
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