[Histonet] immunohistochemistry help please

[Jim Burchette]

Skip the buffer and go in the hematox straight from water. See if that
works.
JB
On Aug 1, 2013 3:20 PM, "Tarantelli, Rebecca Anne" <rat37 <@t> pitt.edu> wrote:

> Hi all,
>
> I am new to this forum, but a friend recommended I send my issue out to
> you for help. Four weeks ago I completed a new protocol for
> Immunohistochemistry (IHC) using protinase K epitope retrieval, and
> monoclonal antibody 3F6. It worked perfectly. Three days later, after 13
> beautiful stains, it just stopped working. The slides will not even take up
> the counter stain - Hematoxylin. I have exchanged my xylene, and tried
> again, and it still is not working. I am guessing since my counter stain
> isn't working, this is an issue in the deparaffinization stage, but I'm
> just not sure.
>
> Please let me know if you have any suggestions!
>
> Thank you
> Rebecca
>
> Rebecca Tarantelli
>
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