[Histonet] RE: Histonet Digest, Vol 113, Issue 30

Dingersoll <@t> aplaboratories.com Dingersoll <@t> aplaboratories.com
Tue Apr 30 13:47:18 CDT 2013


   dingersoll <@t> aplaboratories.com



   


     


   
---   
Subject: [Histonet] RE: Histonet Dige   
From: "Barr,Kaye H" <[1]khbarr <@t> mdanderson.org>
   
Date: Tue, April 30,    
To: "'[2]histonet <@t> lists.utsouthwestern.edu'"
   
<[3]histonet <@t> lists.utsouthwestern.edu>
   

   
What  title  do most use for someone who does gross and is not a P   A?
   

   

   

   

   
-----Original Message-----
   
From:   [[5]mailto:histonet-bounces <@t> lists.utsouthwestern.edu]  O   [6]hi   
Sent: Tuesday, April 30,    
To: [7]histonet <@t> lists.utsouthwestern.edu
   
Subject: Histonet Digest, Vol   

   
Send Histonet mailing list submissions to
      

   
To subscribe or unsubscribe via the Worl   
	[9]http://lists.utsouthwestern.edu/mailman/listinfo/hist   
or, via email, send a message with subject or body 'help' to   
	[10]hist   

   
You can reach the pe   
	[11]histonet-owner <@t> lists.utsouthwestern.edu
   

      specific
      

   

   
Today's To   

   
 1. BIG PROBLEM (Behnaz Sohrab)
   
 2. Re: BIG PR   
 3. Yearly Training (Nancy Schmitt)
   
 4. HIER    
 5. RE: HIER pressure cookers   
 6. RE: HIER pressure cookers.... (Blazek,   
 7. Re: HIER pressure cookers.... (Jan Shivers)
   
 8.   
 9. RE: 5 ga   
   10.   RE:   HIER   pressu   @aplaboratories.com)
   
 11. AW: [Histonet] BIG PROBLEM (Gudrun Lan   
  12. Processor question - alcoholic formalin vs. buffered	forma   lin
   
 (Lake, Kim S)
   
 13. RE: Processor question - alcoholi   
 formalin (Hannen, Valerie)
   
 14.    

   

   
-----------   -----------------------------------------------------------
   

   
Me   
Date: Mon, 29 Apr 2013 10:36:01 -0700
   
From: "Behnaz So   
   
To: <[14]histonet <@t> lists.utsouthwestern.edu>
   
Mes   
Content-Type: text/plain; charset="us-ascii"

   
Good  Morning, We have two Leica H&E stainer( old xt-5010 an   5020). We do use : Gill's 3 for 6 minutes,
   
4% Glacial ACETIC Ac   
Bluing  :  45  sec.  We done this for years!! last few mont   Pantologists  are complaining about GI Biopsies that they have TOO mu   ch blue in cytoplasma and the Mucin is too purple and blue? Please any
   sugg   
Mean  while  we  are trying the NEW kit from Leica for H&E    think  that is TOO pale?any of you has tried this new stain from Leci   a?
   
With much application.
   
Behnaz Sohrab, Los Angeles
   

   

   
------------------------------
   

   
Message: 2
   
Dat   
From: Linda <[16]lmdee1 <@t> yahoo.com>
   
Subject: Re: [Histon   
To: Behnaz Sohrab <[17]SohrabB1 <@t> ah.org>,
   
	"[18]histonet <@t> lists.utsouthwestern.edu"
   
	<histonet <@t> lists.utsouthwester   
Message-ID:
   
	<[19]1367260238.82176.YahooMailN   eo <@t> web164002.mail.gq1.yahoo.com>
   
Content-Type: text/plain; cha   

   
Hi,
   
?
   
I would first check with y   if  they  changed anything.? When som   because the manufacturer have changed.? Thi   stains.
   
?
   
You  may  have to go back to    alcohol,  and  eosin y.? Richard Allen 7   of their staining is excellent in these situat   it back under control.? If you send them slides they   out for you.
   
?
   
If  you are using Leica's selec   lab  out  with because I was heavily sampl   instead  of  the traditional purple.? I do 5 minut   30  dips  in  clarifier  and 1 minute in blue, 55 sec in eos   have  (2)  95s  after.? They also have a tech dept to help.? I have a   Leica autostainer too.
   
?
   
I hope this helps.
   
?
   
Lind   
?
   

   

   
______________________________   
 From: Behnaz Sohrab <[20]Sohrab   
To: [21]histonet <@t> lists.utsouthwestern.edu
   
Sent: Monday, April 29,    
Subject: [Histonet] BIG PROBLEM
   

   

   
G   5020).    
4% Glacial ACETIC Acid 40 seconds   
Bluing  :  45  sec.  We  done  this for years!! last few months our
   Pantol   much blue in cy   any suggestion??
      think   Lecia?
   
Wit   
Behnaz Sohrab, Los Angeles
   

   
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------------------------------
   

   
Message: 3
      
From: Nancy Schmitt <Nancy_Schmitt <@t> pa-ucl.com>
   
Subject: [Histonet] Yearly Training
   
To: "[24]histonet <@t> lists.utsouthwestern.edu"
   
   
Message-ID:
   
	<[26]906B4DA90E   D1DB4DB6C7E94D7CEE6C36813C038E <@t> PEITHA.wad.pa-ucl.com>
   
Content-   

   
Cindy-
   
We  also  ha   standards and includes dir   and maintenance and problem solv   
Systems    covered    are    H&E,    IHC,    Special    s   processing/embedding, Cytology prep, and Misc. Histology.
   
Nanc   
Histology Coordinator
   
Dubuque, IA
   

   
------   

   

   
From:    
Sent: Friday, April 26, 2013 4:49 PM
   
To: '[28]histonet-bounces <@t> lists.utso   
Subject: Yearly training
   

   

   

   
Happy Friday Everyone
   

   
I  just wanted an opinion on what eve   annually.
   
An  example is HIPPA training.   wanted  to  see what everyone else in hi   advance  for  all  responses.  I  hope  everyone has   Finally we are going to have a good weather weekend in We   

   
Cindy
   

   

   

   

   

   
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-----   

   
Message: 4
   
Date: Tue, 30 Apr 2   
From: "k. leigh adams" <[29]k.leigh.adams.865 <@t> gmail.com>
   
Subject:    
To: [30]histonet <@t> lists.utsouthwestern.edu
   
Messag   
	<[31]CAHot8dV4sR0aDAgb9+Ggbd6tH3CbrRbs0TdrB1BU0hob   rHbYNQ <@t> mail.gmail.com>
   
Content-Type: text/plain; charset=ISO   

   
Any   input   as   to   preferred  instruments  would  be  greatly   appreciated...
   

   
Leigh
   

   

   
--------------------   

   
Message: 5
   
Date: Tue, 30 Apr 2013 09:53:50 -0   
From: "Connolly, Brett M" <[32]brett_connolly <@t> merck.com>
   
Subject: RE: [Histonet] HIE   
To: "k. leigh adams" <[33]k.leigh.adams.865 <@t> gmail.com>,
   
	"[34]histonet <@t> lists.utsouthwe   
	<[35]histonet <@t> lists.utsouthwestern.edu>
   
Message-ID:
   
	   <[36]C01C35B84DCDCE49BC60867E87F1C8FE68D4403906 <@t> USCTMXP51015.merck   .com>
   
Content-Type: text/plain; charset="us-ascii"
   

   
Biocare Decloaker here !!
   

   
Brett M. Connolly, Ph.D.
   
P   
Merck & Co., Inc.
   
PO Box    
West Point, PA 19486
   
[37]brett_connolly <@t> merck.com
   
T- 215-652-2501
   
F- 215   

   

   

   

   

   

   
-----Original Mes   
From:                 [38]histonet-bounces <@t> lists.utsouthwestern.edu
   [[39]mailto:histonet-bounces <@t> lists.utso   k. leigh adams
   
Sent: Tuesday, April    
To: [40]histonet <@t> lists.utsouthwestern.edu
   
Subject: [Histonet] HIER p   

   
Any   input   as  to  preferred  instruments  woul   appreciated...
   

   
Leigh
   
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[41]Histonet <@t> lists.utsouthwestern.edu   
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for affiliates is available at
   
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proprietary copyrighted and/or legally privil   solely
   
for the use of the individual or entity n   you are
   
not  the  intended  recipient,  and  have   error,
   
please  notify  us immediately by repl   from
   
your system.
   

   

   


   
------------------------------
   

   
Message: 6
   
Da   
From: "Blazek, Linda" <[44]lblazek <@t> digestivespecialists.c   
Subject: RE: [Histonet] HIER pressure cookers....
   
To   leigh adams"
   
	<[46]k.leigh.adams.865 <@t> gmail.com>,
   "histonet <@t> lists.utsouthwester   
	<[47]histonet <@t> lists.utsouthwestern.edu>
   
Message-ID:
   
	<   xchange.digestivespecialists.com>
   
	
   
Content-Type: text   

   
I second that!
   

   
Linda   
Manager/Supervisor
   
GI Pathology of Dayton
      
Phone: (937) 396-2623
   
Email: lblazek <@t> digestivespecialist   

   

   

   
-----Original Message-----
   
From:   [[50]mailto:histonet-bounces <@t> lists.utsouthwestern.edu]  O   Connolly, Brett M
   
Sent:  Tuesday,  April  30,  2013  9:54  AM
To:  k.  leigh adams;
   [51]histonet <@t> lists.utsouthwestern.edu
   
Subject: RE: [Histonet] HIER   

   
Biocare Decloaker here !!
   

   
B   
Principal Scientist, Imaging Dept.
   
Merc   
PO Box 4, WP-44K
   
West Point, PA 19486
   
<   href="mailto:brett_connolly <@t> merck.com">brett_connolly <@t> merck.com
      
F- 215-993-6803
   

   

   

   

   
   

   
-----Original Message-----
   
From:   [52]histonet-bounces <@t> lists.utsouthwester   lto:histonet-bounces <@t> lists.utsouthwestern.edu]  On  Behalf Of k. leigh
   ad   
Sent: Tuesday, April 30, 2013 9:30 AM
   
To:   [54]histonet <@t> lists.utsouthwestern.edu
Subject:   [Histonet]
   HIER pressure cookers....
   

   
Any   input   a   appreciated...
   

   
Lei   
_______________________________________________
   
Histonet mai   
[55]Histon   
[56]http://lists.utsouthwestern.edu/mailman/li   
Notice:  This  e-mail  message,  together  with  any a   contains   information  of  Merck  &  Co.,  Inc.  (One  Merck  Drive,   Whitehouse  Station,  New  Jersey,  USA  08889), and/or its affiliates
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------------------------------
   

   
Message: 7
      
From: Jan Shivers <shive003 <@t> umn.edu>
   
Subject: Re: [   
To: "Blazek, Linda" <[60]lblazek <@t> digestivespecialists.c   
Cc: "[61]   
	<[62]histonet <@t> lists.utsouthwestern.edu>,	   
	<[63]k.leigh.adams.865 <@t> gmail.com>
   
Message-ID:
   
	<CAEoC1q3tgnQTBbQYuLKzwh2symq+MbYLNf_0PgD18SUo+4+mWg <@t> mail.gmail.
   com   
Content-Type: text/plain; charset=ISO-8859-1
   

   
Thir   

   
Jan S
   

   

   

   

   
On Tue, Apr 30   
[64]lblazek <@t> digestivespecialists.com> wrote:
   
   
> I second that!
   
>
   
> Linda Blazek HT (ASCP)
      
> GI Pathology of Dayton
   
> Dig   
> Phone: (937) 396-2623
   
> Email:    
>
   
>
   
>
   
> -----Original Mes   
> From: [66]histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
   
&   Of Connolly, Brett M
   
&   
> To: k. leigh adams; [68]histonet <@t> lists.utsouthwe   
> Subject: RE: [Histonet] HIER pressure cookers....<   
> Biocare Decloaker here !!
   
>
   
> Brett   
> Principal Scientist, Imaging Dept.
   
>   
> PO Box 4, WP-44K
   
> West Point,    
> [69]brett_conno   
> T- 215-652-2501
   
> F- 215-993-6803
      
>
   
>
   
>
   
>
   
>
   
> -   
> From: [70]histonet-bounces <@t> lists.utsouthwestern.edu    
>  [71]histonet-bounces <@t> lists.utsouthwestern.edu]  On  Behalf Of k.
   leigh    
> Sent: Tuesday, April 30, 2013 9:30 AM
   
> To: histonet <@t> lists.utsouthweste   
> Subject: [Histonet] HIER pressure cookers....
   
&   
>  Any  input  as  to preferred instruments would be greatly appre   ciated...
   
>
   
> Leigh
   
> __________________________   
> Histonet mailing list
   
> [72]Histonet <@t> lists.utsouthwestern   
> [73]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
   
>  Notice:  This  e-mail message, together with any attachments, c   ontains
   
>  information  of Merck & Co., Inc. (One Merck Drive, W   Station, New
   
>  Jersey,  USA  08889),  and/or  its  affiliates   information for
   
> affiliates is available at
      confidential,
   
>  pr   intended solely
      If y   
>  not  the intended recipient, and have received this messag   error, please
   
>  notify  us immediately by reply e-mail and the   your system.
   
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>
   
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> [77]Histonet <@t> lists.utsouthwestern.edu
   
   
>
   

   

   

   
--
   
Jan Shivers
   
Senior Scientist
      
Pathology Teaching Program
   
UMN Ve   
University of Minnesota College of Vet   
1333 Gortner Ave.
   
St. Paul, MN 55108
   
61   
[79]shive003 <@t> umn.edu

   

   
------------------------------
   

   
Message: 8
Date: Tue, 30 Apr 2013 10:36:24 -0400
   
From: "Hannen, Valerie" &l   
Subject: RE: [Histonet] 5 gal specimen container search   
To: 'Jackie O'Connor' <[81]b427297   
	"[82]histonet <@t> lists.utsouthwestern.edu"
   
	<[83]histonet <@t> lists.utsouthwestern.edu&g   
Message-ID:    <450B7A81EDA0C54E97C53D60F00776C3231B3D7C30 <@t> isexsto   re03>
   
Content-Type: text/plain; charset="us-ascii"
   

   
   

   
We  have  ordered  these  from a company by the name of    Medical. The lids are sold separately.
   

   
Valerie
      
-----Original Message-----
   
From: [84]histonet-bounces <@t> lists.utsouthwestern.ed   histonet-bounces <@t> lists.utsouthwestern.edu]   On   Behalf   Of   Jackie
   O'Connor   
Sent: Wednesday, April 24, 2013 3:41 PM
   
To: [86]histonet <@t> lists.utsouthwestern.edu
   
Subject: [Histonet] 5 gal specimen container search
   

   

      leakproof     specimen?
   

   
Ja   

   
_______________________________________________
   
   
[87]Histonet <@t> lists.utsouthwestern.edu
   
[88]http://lists.utsouthwestern.ed   

   
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==   

   

   

   
------------------------------
   

   
Message: 9
   
Date: Tue,   
From: "Shirley A. Powell" <[89]POWELL_SA <@t> mercer.edu>
   
Subject: RE   
To:  "Hannen,  Valerie" &l   'Jackie
   
	O'Connor'	<[91]b427297 <@t> aol.com>,
   "[92]histonet <@t> lists.utsouthwestern.edu"
   
	<[93]histonet <@t> lists.utsouthwestern.edu   
Message-ID:
   
	<9BF995BC0E47744E9673A41486E24EE2579   B14884C@[94]MERCERMAIL.MercerU.loca   
Content-Type: text/plain; charset="us-ascii"
   

      purchased  grea   cheaper than the lab s   
Shirley
   

   

   
-----Original        Message-----<   stonet-bounces <@t> lists.utsouthwestern.edu
   [[96]mailto:histonet-bounces <@t> lists.utsouthwestern   Hannen, Valerie
   
Sent: Tuesday, April 30, 2013    
To: 'Jackie O'Connor'; [97]histonet <@t> lists.utsouthwestern.edu
   
Subject: RE: [H   

   
Jackie...
   

      Medical. T   

   
Valerie
   

   
-----Origin   
From:                 [98]histonet-bounces <@t> lists.utsouthwestern.edu
   [[99]mailto:histonet-bounces <@t> list   Jackie O'Connor
   
Sent: Wednesda   
To: [100]histonet <@t> lists.utsouthwestern.edu
   
Subject: [Histone   

   

   
Anyone  in  histoland     leakproof  bucket,  drum,  pail  for     specimen?
   

   
Jackie O'
   

   
__   
Histonet mailing list
[101]Histonet <@t> lists.ut   
[102]http://lists.utsouthwestern.edu/mailman/listinfo/hist   

   
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=========   

   
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   list
   
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[104]http://lists.utsouthweste   

   

   

   
-----------   

   
Message: 10
   
Date: Tue, 30 Apr 2013 0   
From: <[105]Dingersoll <@t> aplaboratories.com>
   
Subject: RE: [Histonet] HIE   
To:  "Blazek,  Linda"  <[106]lblazek <@t> digestivespecialists.com>,	   "Connolly,
   
	Brett M" <[107]brett_connolly <@t> merck.com>,	"k. leigh adams"
   
	<[108]k.leigh.adams.865 <@t> gmail.com>   southwestern.edu"
   
	<[110]histonet <@t> lists.utsouthwestern.edu>
   
Message-ID:
   
	<[111]20130430074051.073ecbdb5144cf8a0   5e574ee22bfb11a.114d0c0283.wbe <@t> email17.secureserver.net>
   
	
Content-Type: text/plain; charset="utf-8"
   

   

   
 [112]dingersoll <@t> aplaboratories.com   

   

   

   

   

   

   

   

      

   

   
-------- Original Message --------
   

      
From: "Blazek, Linda" <[1]lblazek <@t> digestivespecialist   

   
To: "Connolly, Brett M" <[113]brett_connolly <@t> merck.com>, " ada   

   
<[2]k. "[3][114]histonet <@t> lists.utsouthwestern.edu"
   

   
>&l   

   

   
I second that!
      

   

   
Linda Blazek HT (ASCP)
   

   
GI    

   
Digestive Speciali
   
Phone: (937   

   
Email: [5][116]lblazek <@t> digestivespecialists.com
   

   

   
    

   

   
-----Original Message-----
   

   
Fro   istonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
   
    
Sent: Tuesday, April 30, 2013 9:54 AM
   

   
To   
Subject: RE: [Histonet] HIER pressure cookers... <   

   
Biocare Decloaker here !!
   

   

   
    
Brett M. Connolly, Ph
   
Principal Scientist, Imaging Dept.
   

   
Merck & Co., Inc.
   

   
PO Box 4, WP-44K
   
   
West Point, PA 19486
   

   
[9][119]brett_connolly <@t> merck.com
   

   
T- 215-652-   
F- 215-993-6803
   

   

   

   

   

      

   

   

   

   

   

   

   
----   
From: [10][120]histonet-bounces <@t> lists.utsouthwestern.edu
      

   
Sent: Tu    
To: [12][121]histo   

   
Subject: [His
   

   

   
Any input as to preferred ins appreciated...
   
    

   

   
Leigh
   

   
_________
   
Histonet   

   
[13]Histonet@[122]   
[14][123]http://lists.utsouthwestern.edu/mailman/listinf   o/histonet
   

   
Notice:  This  e-mail  message,  together  with  a   contai  ns  information  of  Merck  &  Co.,  Inc.  (One   Whitehouse
   
 Statio contact information
   
[15   confidential,  proprietary cop   is
   
 i this messag recei   fro
   

   

      

   
________________________________________
   
Histo   

   
[16][125]Histonet <@t> lists.utsouthwestern.edu
   

   
[17]ht   

   

   
________________
   
Histonet   

   
[18]Histonet@[126]   
[19][127]http://lists.utsouthwestern.edu/mailman/li   stinfo/histonet
   

   

   

   

   
References
   

   
 1. 3D"mailto:lbla 2. 3D"[128]mailto:k.leigh.adams.865 <@t> gmail.com"
   
   3.   3D"mailto:h   ost/tmp/3D"m     5.     3D"mailto:lblazek     6.     3D"mailto:h    7.
   3D"[130]mailto:histonet-bounces <@t> list   
 8. 3D"[131]mailto:histonet <@t> lists.utsouthwestern.edu"
   
  9   ="[132]mailto:histonet-bounces@   
 12. 3D"mailto:histonet <@t> lists.ut 13.    ts.utsouthwestern.edu"
   
 14.
   3D"[134]lists.utsouthwestern.edu/mailman/list">http://[135]lists.utsou
   thwestern.edu/mailman/list  15.   16.   3D"mailto:Histonet <@t> lists.   17.   file://localhost/tmp/3D"   18.
   ="mailto   
     19.     3D"[137]lists.utsouthwestern.edu/mailman/listinfo">ht   tp://[138]lists.utsouthwestern.edu/mailman/l   

   
------------------------------
   

   
Messa   
Date: Tue, 30 Apr 2013 17:15:01 +0200
   
From: "Gudrun Lang   
Su   
To: "'Behnaz Sohrab'" <[140]SohrabB1 <@t> ah.org>
   
Cc: [141]histonet <@t> lists.utsouthwestern.edu
   
Message-ID: <000301ce45b5$7d292a50$777b7ef0$@[142]gmx.at>
   
Content-Type: text/plain;	charset="iso-885   

   
Too much blue is mostly a problem of too less red.
   
   

   
My  perso   also rather
   
p   
Gudrun
   

   

   
-----Urspr?ngliche Nachricht-----
      
[[144]mailto:histonet-bounces <@t> lists.utsouthweste   Auftrag von Behnaz
   
Sohrab
   
Gesendet: Montag, 29.    
An: [145]histonet <@t> lists.utsouthwestern.edu
   
Betreff: [Histonet] BIG PR   

   
Good  Morning, We have two Leica H&E stainer( old xt-   5020). We
   
do  use  :  Gill's  3  for  6  minutes, 4% Glacial AC   seconds Bluing :
   
45 sec. We done this for years!! last f   are
   
complaining  about  GI  Biopsies  that the   cytoplasma and
   
the Mucin is too purple and bl   
Mean  while  we  are  trying the NEW kit from   think that is
   
TOO pale?any of you has tried   
With much application.
   
Behnaz Sohra   

   

   

   

   

   
------------------   

   
Message: 12
   
Date: Tue, 30 Apr 2013 15:30:33   
From: "Lake, Kim S" <[146]   
Subject: [Histonet] Processor question - a   
	buffered	formalin
   
To: "[147]histonet <@t> lists.utsouthwestern.edu"
   
	<[148]histo   
Message-ID:
   
	<[149]F3181E1428AD274EAEF67664C5B662AB25827126 <@t> itsnt443.iowa.uio
   wa.edu><   

   
I  attende   (which  was  fantastic)  an   workshop.  At  this  workshop  I reali   been having with sectioning could be due to    

   
Before  microtomy  we  have to soak our b   and  I  suspect  that  this  is  because we are over deh   tissues  in  the  during  processing.  Our first solution, which t   cassettes  sit  in  for  about  8 hours until the overnight processing
   begins   900ml water, and   that has been chuggi   

   
The  reason  we  use  alcoholic  f   buffered  formalin  has  been  lost  to  the mists   wondering  what you all use as a holding solution on you   and why.
   

   
Thanks!
   

   
Kim Lake
   
Labo   
Oral Pathology Laboratory
   
University of Iowa Col   
Phone (319) 384 4433
   
Fax (319) 353 5569
      

   

   
------------------------------
   

   
Message   
Date: Tue, 30 Apr 2013 11:40:53 -0400
   
From: "Hannen, Valer   
Subject:  RE:  [Histonet] Processor question - alco   vs.
   
	buffered	formalin
   
To: "'Lake, Kim S'" &   
   
	<[153]histonet <@t> lists.utsouthwestern.edu>
   
Message-ID: <   ;450B7A81EDA0C54E97C53D60F00776C3231B3D7C33 <@t> isexstore03>
   
Content-T   

   
  We  use  10  %  Neutral  B   fixative and gives us really great fixa   
It does sound like you are overly dehydrating your tissues.
      
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
   
Histology Section C   
Parrish Medical Center
   
951 N. Washington Ave.
   
Titusv   
Phone:(321) 268-6333 ext. 7506
   
Fax: (321) 2   
[154]valerie.hann   

   

   

   
-----Original Message-----   
From:        [155]h   [[156]mailto:histonet-bounces <@t> lists.utsouthwester   Lake, Kim S
   
Sent: Tuesday, April 30, 2013 11:   
To: [157]histon   
Subject:  [Histonet]  Processor questio   buffered formalin
   

   
I  attended  the  Tr   (which  was  fantastic)  and  sat  in   workshop. At this workshop I realized that   having with sectioning could be due to our proc   

   
Before  microtomy  we  have to soak our blocks fo   and  I  suspect  that  this  is  because  we are over dehydrating   tissues in the during processing. Our first solution, which the casse   ttes  sit  in for about 8 hours until the overnight processing begins,
   is  alc   water, and 3300ml    has been chugging along   

   
The  reason  we  use  alcoholic  formalin     buffered  formalin  has  been  lost  to the mists of time   wondering  what you all use as a holding solution on your proces   and why.
   

   
Thanks!
   

   
Kim Lake
   
Laboratory M   
Oral Pathology Laboratory
   
University of Iowa College of    
Phone (319) 384 4433
   
Fax (319) 353 5569
   

   
   
Histonet mailing list   
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[159]http://lists.utsouthwestern.edu/mailman/listinfo/hi   

   
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-----------------   

   
Message: 14
   
Date: Tue, 30 Apr 2013 16:31:4   
From: "Margiotta-Watz, Michele" <[160]MMargiotta <@t> bmhmc.org>
   
Subject: [Histonet] casse   
To: "'[161]histonet <@t> lists.utsouthwestern.edu'"
   
	<[162]histonet <@t> lists.utsouthwestern.edu>   
Message-ID:
   
	<[163]230D0B9EC57D7A45A7A186C6AB4C7ABC   296F39AA <@t> BMH-EXCHANGE-02.BMHMC.ORG>
   
Content-Type: text/plain;    

   
Hi All,
   
We  are  looking  to  purchase    some  feedback  on  which ones you might re   difference  between  Thermo's  Printmate and Microwr   the  Printmate  is  just a newer version. Your input would be   appreciated.
   

   
Thanks,
   
Michele
   
Histology Su   
BMHMC
   
631-654-7192
   
DISCLAIMER:
   

   
This   are
   
in   they
   
a   by
   
t   recipient
   
   intended
   
r   and
   
   this
      in
      call
   
Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
   


   
------------------------------
   

   
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End of Histonet Digest, Vol 113, Issue 30
   
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