[Histonet] Isopentane storage-the polling is open

[Maria Mejia]

Hello Paula,

At my previous lab at UCSF, we often had a lot of large & small tissue to freeze &
therefore we used quite a bit of liquid nitrogen.  We would get our LN2 from a local
vendo, & was delivered in a low pressure 230 tall slender LN2 tank.  The tank 
had a nozzle attached for easy & safe dispensing. We then dispensed LN2 into 
1 and 2 liter Dewar flasks.  These flasks were keep at room temp (under the hood), 
ready to use during freezing.

This 230 liter tank was strapped stored in a corner of the lab (room temp) & the
LN2 inside the tank would last about 3 weeks.  The LN2 vendor automatically 
made delivers of LN2 to our lab - once a month & cost under $100/month.

This local LN2 vendor, also carries smaller tanks filled with LN2 which can be 
stored at room temp., with dispensing nozzles.  Paula, this is another option for
you - but you'd still need to purchase a Dewar flask to use during freezing.

Hope all is well.

Best
Maria Mejia
Affymetrix, Inc


On Apr 16, 2013, at 10:35 AM, Paula Sicurello wrote:

> Hello HistoNetters,
> 
> I have been having a discussion with my boss about whether or not to store
> the isopentane (used for freezing muscle biopsies) in the refrigerator. (An
> expensive, explosion proof one that I would have to buy.)
> 
> Her only experience is with the isopentane stored in the refrigerator, my
> experiences are with storing it at room temperature.
> 
> What do you all do?  Room temp or refrigerated?
> 
> Any and all comments are greatly appreciated.
> 
> Thanks!
> 
> Paula
> 
> -- 
> Paula Sicurello, HTL (ASCP)
> Supervisor, Clinical Electron Microscopy Laboratory
> Duke University Health System
> Rm.#251M, Duke South, Green Zone
> Durham, North Carolina 27710
> P:  919.684.2091
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