[Histonet] RE: Histonet Digest, Vol 113, Issue 16

Zimmerman, Billie BZIMMERM <@t> gru.edu
Tue Apr 16 13:57:02 CDT 2013


Thanks to Deloris for taking the time to attend our GSH symposium and her crochet bikini was beautiful! Thanks also to Janet Hobbs for bringing her crochet bikini.  The speakers as well as the vendors were wonderful.  It was a packed house and a lot of fun.  Who says learning can't be fun?!
It was a Class Act and we're looking forward to next year.  Thanks again to everyone that attended.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, April 16, 2013 1:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 113, Issue 16

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Today's Topics:

   1. Blades (Hale, Meredith)
   2. Question regarding appropriate use of histonet (Henry Chidgey)
   3. Thermohistoscreen Cassettes (DeMint, Valerie S)
   4. Bone, Biomaterials & Medical Device Implants Histology Event
      In Cambridge, MA - May 4th, 2013 (Jack Ratliff)
   5. (no subject) (Karla Arrington)
   6. Glucose Oxidase blocking method (Long, Denise)
   7. Home based slide interpretation (Jennifer MacDonald)
   8. Immunohistochemistry for Adipose tissue macrophages in    humans
      (khadija irfan)
   9. Re: Home based slide interpretation (Rene J Buesa)
  10. Picrosirius red on cryosections (Laura Avogaro)
  11. RE: Picrosirius red on cryosections (Elizabeth Chlipala)
  12. RELIA Hot Job Alert 4-16-2013 Exciting Opportunities in
      NC.VA, GA, CA, CO, IL, TX and NM (Pam Barker)
  13. SP and CGRP antibodies (Dessoye, Michael J)
  14. GSH Symposium (Deloris Carter)
  15. RE: GSH Symposium (Shirley A. Powell)


----------------------------------------------------------------------

Message: 1
Date: Mon, 15 Apr 2013 17:39:26 +0000
From: "Hale, Meredith" <mhale <@t> MiracaLS.com>
Subject: [Histonet] Blades
To: "Histonet <@t> lists.utsouthwestern.edu"
        <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <0E828EC51C7CC445A51E53F81B64E8C721A8B5 <@t> s-irv-exchmb.PathologyPartners.intranet>

Content-Type: text/plain; charset="us-ascii"

I would like some feedback from you on the types of blades those of you who cut GI biopsies prefer ? Do you see differences with chatter on different blades. Any feedback is appreciated . Thanks !

Meredith Hale HT  (ASCP)cm
Director External Sales Support

Miraca Life Sciences
6655 North MacArthur Blvd.
Irving , Texas 75039
Office: 214-596-2219
Cell: 469-648-8253
Fax: 1-866-688-3280
mhale <@t> miracals.com<mailto:mhale <@t> miracals.com<mailto:mhale <@t> miracals.com%3cmailto:mhale <@t> miracals.com>>



------------------------------

Message: 2
Date: Mon, 15 Apr 2013 12:48:46 -0500
From: "Henry Chidgey" <hchidgey <@t> earthlink.net>
Subject: [Histonet] Question regarding appropriate use of histonet
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <005801ce3a01$7c705d00$75511700$@net>
Content-Type: text/plain;       charset="us-ascii"

Howdy Folks,

As I am sure you can tell we are subscribers to histonet and appreciate the generosity of members in helping solve the occasional problem we encounter.
We are in need of finding either an individual or lab in our area to perform mammal root sectioning and slide preparation for the forensic mammal aging business we operate located near Burnet, Texas in the Texas Hill Country. I would like to reach out to your subscriber base to see if anyone is interested in doing some part time work for us or know someone who might be.
Please let me know if that would be an appropriate use of the site.

Thanks,

Henry Chidgey

CoFounder



See what folks are saying about us <http://www.deerage.com/testimonials.htm>


Wildlife Analytical Laboratories

 www.DeerAge.com <http://www.deerage.com/>

 PO Box 295

 1303 CR 118 B

 Burnet, Texas 78611

(512) 756-1989

(512) 663-9476 Cell

Serving Wildlife Stewards, Ranchers & Hunters Worldwide

Customer Service and Quality of Analysis are our Priority and Passion

Facebook <http://www.facebook.com/DeerAge>   Eagle Soybeans
<http://deerage.com/eagle_seed/>







------------------------------

Message: 3
Date: Mon, 15 Apr 2013 13:09:10 -0500
From: "DeMint, Valerie S" <Valerie.DeMint <@t> phci.org>
Subject: [Histonet] Thermohistoscreen Cassettes
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <657B95ED46A98B4D9F44EFC67EDCAADD07477024 <@t> RWDEX3.WHS.phci.org>
Content-Type: text/plain;       charset="us-ascii"

Is anyone waiting for their backorder of Thermo Histoscreen Cassettes?
We no longer use this cassette and I am trying to sell my remaining inventory.  I have several colors available.  Write if you want more information :-)



Valerie DeMint, HT(ASCP)

Waukesha Memorial Hospital

Waukesha WI



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------------------------------

Message: 4
Date: Mon, 15 Apr 2013 14:14:28 -0400
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: [Histonet] Bone, Biomaterials & Medical Device Implants
        Histology Event In Cambridge, MA - May 4th, 2013
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Cc: Jack Ratliff <jratliff <@t> ratliffhistology.com>,       Jack Ratliff
        <ratliffjack <@t> gmail.com>
Message-ID: <BLU167-W18C069913A36CCBAC3CD9FAECC0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

If you are currently working with bone, biomaterials or medical device implants, you won't want to miss this specialty histology event http://www.polysciences.com/Interactive-Histology-Forum-About/185/ sponsored by Polysciences Inc. and supported with six (6) continuing education units (CEU's) by the National Society for Histotechnology! There is still time to pre-register online http://events.r20.constantcontact.com/register/event?llr=gqebcrbab&oeidk=a07e6rs5o33629b055e or onsite May 4th at the Hyatt Regency Cambridge http://www.polysciences.com/Interactive-Histology-Forum-Hotel-Travel/189/.

Topics of discussion will include:

A Closer Look at Fixation, Decalcification and Processing Techniques for Paraffin Embedded SpecimensPresented by Robert A. Skinner, HTL (ASCP), Director of Laboratory Resources, Center for Orthopaedic Research, University of Arkansas for Medical Sciences

Histology Technique Selection and Challenges for Biomaterial and Medical Device Implanted TissuesPresented by Philip Seifert, MS, HTL (ASCP), Principal Scientist, CBSET, Inc.

Acrylic Resins: A Practical Approach for Demonstrating Undermineralized Bone, Biomaterials and Medical Device ImplantsPresented by Jack L. Ratliff, BA, Owner, Ratliff Histology Consultants & Chairman, Hard Tissue Committee National Society for Histotechnology

The Use Of A Non-Contact Laser To Collect Thin Sections From A Variety of Soft And Hard Histological Tissues, Synthetic Bio-Resorbable Materials, and Tissues Containing Non-Resorbable Medical Device ImplantsPresented by Heiko Richter, Ph.D., Sales & Product Management, Rowiak GmbH

Introduction to Routine Staining and Special Staining with Plastic Resin TechniquesPresented by Valantou Grover, HT (ASCP) HTL, PA, Product Line Manager, Business Manager, Histologist at Polysciences, Inc.

If you have any questions, please feel free to contact myself or Ashley Gidzinski (Ashley.Gidzinski <@t> polysciences.com). We look forward to seeing you in Cambridge, MA and May the 4th be with you!

Best Regards,

Jack

Owner/Histologist, Ratliff Histology Consultants, LLC
Chairman, Hard Tissue Committee - National Society for Histotechnology

------------------------------

Message: 5
Date: Mon, 15 Apr 2013 13:04:21 -0700 (PDT)
From: Karla Arrington <freckles9660 <@t> yahoo.com>
Subject: [Histonet] (no subject)
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1366056261.91439.YahooMailNeo <@t> web121901.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=us-ascii

How do I boost the power of mucicarmine for staining goblet cells? I have opened a new lot/bottle and have used full strength but it is not staining the cells.
Thanks,
Karla

------------------------------

Message: 6
Date: Mon, 15 Apr 2013 21:18:07 +0000
From: "Long, Denise" <denise.long <@t> uconn.edu>
Subject: [Histonet] Glucose Oxidase blocking method
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F72A572 <@t> MAILD.grove.ad.uconn.edu>
Content-Type: text/plain; charset="us-ascii"

Hello,
I'm looking for updated information on the Glucose Oxidase blocking method. In particular, what reagents are being used now that Sigma's G5250 is no longer available.  Does anyone know of a company that has this as a kit?
Thanks,
Denise

Denise M. Long, MS, HTL (ASCP), QIHC
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851



------------------------------

Message: 7
Date: Mon, 15 Apr 2013 15:12:23 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: [Histonet] Home based slide interpretation
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <OFAE011749.9E823DD0-ON88257B4E.0079E2F2-88257B4E.0079EC21 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"

Does anyone know of any regulations against a Pathologist reading slides from home?  If so would you be able to cite the specific regulation?
Thanks,
Jennifer

------------------------------

Message: 8
Date: Mon, 15 Apr 2013 22:19:39 -0700 (PDT)
From: khadija irfan <khadijairfan <@t> yahoo.com>
Subject: [Histonet] Immunohistochemistry for Adipose tissue
        macrophages in  humans
To: "Histonet <@t> lists.utsouthwestern.edu"
        <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1366089579.51528.YahooMailNeo <@t> web122204.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1



Hello All
?
I am a clinical researcher so my questions may be somewhat naive, but I would be extremely grateful for any help.
I want to look at adipose tissue macrophage polarity by immunohistochemistry for activation markers. I have the following questions:
1. How much tissue sample is required. I want to use subcutaneous and omental fat samples.
2. How should I transport and store my samples. How long can I store them? I anticipate that I would be collecting samples over 2-3 months.
3. If I do H&E staining first, can I do immunohistochemistry on the same slide? Can I do successive markers on the same slide? How many markers can I use it for?
?
Thanks
?
Khadija

------------------------------

Message: 9
Date: Tue, 16 Apr 2013 06:38:26 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Home based slide interpretation
To: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1366119506.58355.YahooMailNeo <@t> web163104.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

The only regulation about reading slides "from home" refers to cytotechnologists because they have a limit of allowed cases/day that has to be controlled?at the usual work setting, but no such regulation exists for pathologists and I really?do not think that a pathologist is limited to read slides from home.
The only possible objection would be a pathologist working in the UK because, under the NHS regulations,? pathologists have norms?about the?time per type of case and its complexity.? This time/case was designed to assure that the pathologist takes enough time for the diagnosis which, according with the system design, assures quality of work, and also links work-to-remuneration rates. Under those circumstances perhaps the pathologists would have to justify how much time they have used (to be paid for) per different cases, and that would require a work based control.
Fortunately such a system does not exist here (yet!).
Ren? J.

From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Monday, April 15, 2013 6:12 PM
Subject: [Histonet] Home based slide interpretation


Does anyone know of any regulations against a Pathologist reading slides
from home?? If so would you be able to cite the specific regulation?
Thanks,
Jennifer
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 10
Date: Tue, 16 Apr 2013 16:30:14 +0200 (CEST)
From: "Laura Avogaro" <avogaro <@t> science.unitn.it>
Subject: [Histonet] Picrosirius red on cryosections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <2828.192.168.178.78.1366122614.squirrel <@t> www.science.unitn.it>
Content-Type: text/plain;charset=iso-8859-1

Hi everyone!

I would like to stain cryosections of human atrial tissue with Picrosirius
Red in order to quantify collagen fibers. Cryosections are obtained by a
sample embedded in OCT compound without any fixative.
Does anyone can suggest me a protocol to perform this staining?
Thanks in advance

Laura


Laura Avogaro
Center for Biomedical Technologies (BIOTECH)
University of Trento
Via delle Regole, 101 38123 Mattarello (TN) ? Italy
Tel:  +39 0461 283425












------------------------------

Message: 11
Date: Tue, 16 Apr 2013 08:42:25 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: RE: [Histonet] Picrosirius red on cryosections
To: Laura Avogaro <avogaro <@t> science.unitn.it>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <14E2C6176416974295479C64A11CB9AE016411AC2AE3 <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"

Laura

This type of analysis does not need to be completed on frozen sections it can be performed on routine 10% NBF fixed paraffin sections.  There are protocols on line on the website stains file.

Here is the link  http://stainsfile.info/StainsFile/stain/conektv/puchtler_psr.htm

 I would let the frozen sections air dry for at least 30 minutes and then post fix in 10% NBF for about 10 minutes and then start this procedure.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laura Avogaro
Sent: Tuesday, April 16, 2013 8:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Picrosirius red on cryosections

Hi everyone!

I would like to stain cryosections of human atrial tissue with Picrosirius Red in order to quantify collagen fibers. Cryosections are obtained by a sample embedded in OCT compound without any fixative.
Does anyone can suggest me a protocol to perform this staining?
Thanks in advance

Laura


Laura Avogaro
Center for Biomedical Technologies (BIOTECH) University of Trento Via delle Regole, 101 38123 Mattarello (TN) - Italy
Tel:  +39 0461 283425










_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 12
Date: Tue, 16 Apr 2013 11:32:41 -0400
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Hot Job Alert 4-16-2013 Exciting
        Opportunities in        NC.VA, GA, CA, CO, IL, TX and NM
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0b2801ce3ab7$a2bec300$e83c4900$@earthlink.net>
Content-Type: text/plain;       charset="us-ascii"

Hi Histonetters,
I hope you enjoyed a beautiful Spring weekend.
I wanted to send a quick note to tell you about the positions that I am
working on and am most excited about.
Why am I excited about these positions?  Because these clients offer
excellent compensation, benefits, relocation assistance and most importantly
they are ready to interview and hire ASAP.
All of these positions are full time and permanent
The histology positions that I am most excited about are located in these
areas:
Charlotte, NC
Salem, VA
Staunton, VA
Atlanta, GA
Long Beach, CA
Denver, CO
Chicago, IL
Springfield, IL
Tyler, TX
Waco, TX
Roswell, NM
Long Island, NY
Syracuse, NY
Louisville, KY
East of Columbus, OH
If you think you or someone you know might be interested in any of these
opportunities or would like to talk about a job search in another area,
please contact me.
If I place someone you refer You will earn a referral fee.

I can be reached toll free at the office at 866-607-3542 or
relia1 <@t> earthlink.net or you can always catch me on cell via call or text at
407-353-5070.   Thanks-Pam

Thank You!

Pam Barker
President
RELIA
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1 <@t> earthlink.net
www.facebook.com  search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia


------------------------------

Message: 13
Date: Tue, 16 Apr 2013 11:53:45 -0400
From: "Dessoye, Michael J" <mjdessoye <@t> commonwealthhealth.net>
Subject: [Histonet] SP and CGRP antibodies
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <E2547E1CD0EE324488A2940994571EFA0549DC25 <@t> WVHCS-EXCHANGE.wvhcs.com>
Content-Type: text/plain;       charset="us-ascii"

Hello everyone,

Is anyone out there using a commercially available antibody for SP
(Substance P) and CGRP (Calcitonin gene-related peptide)?  If so, where
do you obtain it?

Thanks!

Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

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------------------------------

Message: 14
Date: Tue, 16 Apr 2013 11:30:26 -0500
From: Deloris Carter <delsuec <@t> gmail.com>
Subject: [Histonet] GSH Symposium
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <CAJ6XiEFKfWWYROV2Hbb03aobEA4Cyy4bye+Y38_Z9kPs5JxYvw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

I'd like to say thank you to everyone at the GSH Symposium for making this
"Yankee" feel welcome. I had a great time and found the sessions very
helpful. Thanks again for letting me crash the party!
Deloris Carter


------------------------------

Message: 15
Date: Tue, 16 Apr 2013 12:52:11 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: RE: [Histonet] GSH Symposium
To: Deloris Carter <delsuec <@t> gmail.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <9BF995BC0E47744E9673A41486E24EE25799E77BB2 <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="us-ascii"

GSH would like to thank you for coming.  Glad you had a good time and we will let you know when the next meeting is and the dates as soon as we get them.  Crash our party anytime.

Shirley Powell

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Deloris Carter
Sent: Tuesday, April 16, 2013 12:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] GSH Symposium

I'd like to say thank you to everyone at the GSH Symposium for making this "Yankee" feel welcome. I had a great time and found the sessions very helpful. Thanks again for letting me crash the party!
Deloris Carter
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 113, Issue 16
*****************************************
Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM <@t> gru.edu. Please update your address book to reflect this change.



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