[Histonet] Re: Histonet Digest, Vol 106, Issue 34
Jeanne Clark
paintedsplashes <@t> yahoo.com
Thu Sep 27 12:10:07 CDT 2012
Hello all,
Anyone arriving in Vancouver airport around 4 to 5pm that would like to share a limo over to the convention center arear? If 3 or 4 of us can combine, its actually cheaper than a cab? And I'm sure a much more enjoyable ride!
Jeanne Clark (SFO to Vancouver)
From: "histonet-request <@t> lists.utsouthwestern.edu" <histonet-request <@t> lists.utsouthwestern.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, December 31, 1969 4:00 PM
Subject: Histonet Digest, Vol 106, Issue 34
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When replying, please edit your Subject line so it is more specific
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Today's Topics:
1. Have Blocks Will Cut (Contact HistoCare)
2. ShurMount coverslipper (Charles, Roger)
3. Question (Richard Cartun)
4. Ferret lungs (Blair, Paula)
5. Come and visit the Legislative Committee in Vancouver!
(Colleen Forster)
6. RE: softening agent for tissues embedded in paraffin blocks
(Debbie Faichney)
7. Re: Ferret lungs (Rene J Buesa)
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Message: 1
Date: Wed, 26 Sep 2012 12:17:17 -0500
From: Contact HistoCare <contact <@t> histocare.com>
Subject: [Histonet] Have Blocks Will Cut
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2EEBC9DB-153F-4BB0-9F92-9587F3AC321F <@t> histocare.com>
Content-Type: text/plain; charset=us-ascii
Thanks to everyone who inquired about contracting my services and gave me an opportunity to explain the value I add. This is a great opportunity for those involved and it keeps the stress to a minimum and puts people in a better mood since the individual workload is lighter and you don't feel like a machine.
For the next few months, my reach will remain in the southern states unless I'm compelled to do otherwise :).
Please contact me if you need some relief in your lab, I know many do but may not want to admit it :)
Thanks again histonet
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Message: 2
Date: Wed, 26 Sep 2012 13:58:25 -0400
From: "Charles, Roger" <rcharles <@t> pa.gov>
Subject: [Histonet] ShurMount coverslipper
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3809C163DC1DA54AA534B3C7794D07B6CA7382D124 <@t> ENHBGMBX01.PA.LCL>
Content-Type: text/plain; charset="us-ascii"
Hello,
Does anyone have experience with the ShurMark glass coverslipper. We are looking at purchasing one and I would like to hear pros and cons.
Thanks
Roger
Roger Charles| Microbiologist II
Pennsylvania Veterinary Laboratory
2305 North Cameron Street | Harrisburg, PA 17110
Phone: 717.787.8808 | Fax: 717.772.3895
www.agriculture.state.pa.us<http://www.agriculture.state.pa.us/>
------------------------------
Message: 3
Date: Wed, 26 Sep 2012 16:02:38 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] Question
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5063271F.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII
I was asked by a colleague if there is testing for "fibrinogen storage disease" that can be performed on fixed tissue. I told her that I did not know, but I would ask the experts. Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-2204 Fax
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Message: 4
Date: Wed, 26 Sep 2012 21:58:55 +0000
From: "Blair, Paula" <Paula.Blair <@t> cchmc.org>
Subject: [Histonet] Ferret lungs
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CC88F2DE.1504%paula.blair <@t> cchmc.org>
Content-Type: text/plain; charset="us-ascii"
I am sectioning ferret lungs and am having problems with tearing and lines because the bronchioles and blood vessels are so thick , similar to trachea. Has anyone sectioned these animals and can give suggestions on soaking or anything that will help get a smooth section.
------------------------------
Message: 5
Date: Wed, 26 Sep 2012 17:44:48 -0500
From: Colleen Forster <cforster <@t> umn.edu>
Subject: [Histonet] Come and visit the Legislative Committee in
Vancouver!
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <50638560.6070209 <@t> umn.edu>
Content-Type: text/plain; charset="iso-8859-1"
Greetings to everyone on behalf of the Legislative Committee.
I am formally inviting each of you to stop by our booth #625
while you are browsing about the product hall. We would love
to meet you. This year we will have a couple different flag
designs to look at or maybe you have another idea to
present...come and vote and get your button!
Our committee meets on Saturday September 29th at noon in room
208/209. Grab your lunch and join us. The more discussion we
have the better!
See you soon,
Colleen Forster Legislative Chair.
------------------------------
Message: 6
Date: Thu, 27 Sep 2012 09:40:10 +0100
From: Debbie Faichney <d.a.faichney <@t> stir.ac.uk>
Subject: RE: [Histonet] softening agent for tissues embedded in
paraffin blocks
To: Jose Luis Palazon Fernandez <jluis.palazon <@t> icman.csic.es>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8ED3F2CA5B78E142B8193376C57330F8FA2EA21FBE <@t> EXCH2007.ad.stir.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
Hello Jose,
We routinely surface decalcify the trimmed/faced block for one hour with a commercial product (Rapid Decalcifier or RDC, Cellpath) which contains Hydrochloric acid. Works a treat! Alternatively we have been known to use a splash of laundry fabric softener in the soaking water for IHC sections.
Which species of fish are you sectioning? This can make a huge difference to the timing in either solution.
Best regards
Debbie Faichney
Histopathology
Institute of Aquaculture
University of Stirling
Stirling, FK9 4LA
UK
.·´¯`·...¸><((((º>`·.¸¸.·´¯`·.¸.·´¯`·...,><((((º>
.·´¯`·.......¸><((((º>`·....¸¸...·´¯`·.¸.·´¯`·...,..><((((º>
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jose Luis Palazon Fernandez
Sent: 26 September 2012 10:56
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] softening agent for tissues embedded in paraffin blocks
Dear List-members
I'm trying to cut some fish muscle samples (along with skin and some bone tissues) embedded in paraffin (fixed in Bouin's fixative and decalcified with EDTA). I find it very difficult to cut these blocks and I would like to know, based in your experience, what tissue softening agent do you recomend to solve this problem. Many thanks in advance
greetings,
José Luis
Dr. José Luis Palazón Fernández
Instituto de Investigaciones Científicas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC Campus universitario Rio San Pedro, 11510, Puerto Real, Cádiz, España
email: jluis.palazon <@t> icman.csic.es; jose.palazon <@t> ne.udo.edu.ve
tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100 _______________________________________________
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------------------------------
Message: 7
Date: Thu, 27 Sep 2012 07:05:05 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Ferret lungs
To: "Blair, Paula" <Paula.Blair <@t> cchmc.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1348754705.63464.YahooMailNeo <@t> web163102.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
You have to assure a perfect infiltration. Cartilage needs that. Also you will have to "play" with sectioning speed, type of blade (knife) and sectioning angle.
René J.
________________________________
From: "Blair, Paula" <Paula.Blair <@t> cchmc.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, September 26, 2012 5:58 PM
Subject: [Histonet] Ferret lungs
I am sectioning ferret lungs and am having problems with tearing and lines because the bronchioles and blood vessels are so thick , similar to trachea. Has anyone sectioned these animals and can give suggestions on soaking or anything that will help get a smooth section.
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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